ARG55499

anti-DDAH2 antibody

anti-DDAH2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Neuroscience antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes DDAH2
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name DDAH2
Antigen Species Human
Immunogen Recombinant protein of Human DDAH2
Conjugation Un-conjugated
Alternate Names G6a; DDAH; NG30; DDAHII; HEL-S-277; N(G),N(G)-dimethylarginine dimethylaminohydrolase 2; DDAH-2; Dimethylarginine dimethylaminohydrolase 2; EC 3.5.3.18; DDAHII; Dimethylargininase-2; Protein G6a; S-phase protein

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control BT474
Observed Size ~ 31 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 23564 Human DDAH2

GeneID: 294239 Rat DDAH2

GeneID: 51793 Mouse DDAH2

Gene Symbol DDAH2
Gene Full Name dimethylarginine dimethylaminohydrolase 2
Background This gene encodes a dimethylarginine dimethylaminohydrolase. The encoded enzyme functions in nitric oxide generation by regulating the cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. The protein may be localized to the mitochondria. Alternative splicing resulting in multiple transcript variants. [provided by RefSeq, Dec 2014]
Function Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. [UniProt]
Research Area Neuroscience antibody
Calculated MW 30 kDa

Images (2) Click the Picture to Zoom In

  • ARG55499 anti-DDAH2 antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG55499 anti-DDAH2 antibody.

  • ARG55499 anti-DDAH2 antibody WB image

    Western blot: BT474 cell lysate stained with ARG55499 anti-DDAH2 antibody.