ARG56245
anti-DARS antibody
anti-DARS antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DARS |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DARS |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human DARS |
| Conjugation | Un-conjugated |
| Alternate Names | aspRS; AspRS; Cell proliferation-inducing gene 40 protein; Aspartyl-tRNA synthetase; EC 6.1.1.12; Aspartate--tRNA ligase, cytoplasmic; HBSL |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | MCF7 |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DARS |
| Gene Full Name | aspartyl-tRNA synthetase |
| Background | This gene encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA(Asp). Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014] |
| Function | Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. [UniProt] |
| Calculated MW | 57 kDa |
Images (3) Click the Picture to Zoom In
-
ARG56245 anti-DARS antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver injury stained with ARG56245 anti-DARS antibody at 1:100 dilution.
-
ARG56245 anti-DARS antibody WB image
Western blot: MCF7 cell lysate stained with ARG56245 anti-DARS antibody.
-
ARG56245 anti-DARS antibody IP image
Immunoprecipitation: 200 µg extracts of 293T cells were immunoprecipitated and stained with ARG56245 anti-DARS antibody at 1:1000 dilution.
