ARG62969
anti-Cytokeratin (pan) antibody [C-11]
anti-Cytokeratin (pan) antibody [C-11] for CyTOF®-candidate,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Mammal
Signaling Transduction antibody; Carcinoma Marker antibody
Overview
| Product Description | Mouse Monoclonal antibody [C-11] recognizes Cytokeratin (pan) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Mamm |
| Tested Application | CyTOF®-candidate, FACS, ICC/IF, IHC-P, IP, WB |
| Specificity | The clone C-11 reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | C-11 |
| Isotype | IgG1 |
| Target Name | Cytokeratin (pan) |
| Antigen Species | Human |
| Immunogen | Keratin-enriched preparation from human epidermoid carcinoma cell line A431. |
| Conjugation | Un-conjugated |
| Alternate Names | 67 kDa cytokeratin; KRT1A; EPPK; Keratin, type II cytoskeletal 1; EHK1; K1; Keratin-1; CK1; Cytokeratin-1; Hair alpha protein; Type-II keratin Kb1; CK-1; NEPPK; EHK |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRT1 |
| Gene Full Name | keratin 1, type II |
| Background | Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. |
| Function | May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30319 Carcinoma / Sarcoma Antibody Duo Related products: Cytokeratin antibodies; Cytokeratin Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| Research Area | Signaling Transduction antibody; Carcinoma Marker antibody |
| Calculated MW | 66 kDa |
| PTM | Undergoes deimination of some arginine residues (citrullination). |
Images (3) Click the Picture to Zoom In
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ARG62969 anti-Cytokeratin (pan) antibody [C-11] WB image
Western blot: 30 µg of MCF-7 cell lysate stained with ARG62969 anti-Cytokeratin (pan) antibody [C-11] at 1:500 dilution.
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ARG62969 anti-Cytokeratin (pan) antibody [C-11] ICC/IF image
Immunofluorescence: Rat endometrial epithelial (REE) cultured cells fixed with neutral buffered formalin for 10 min, and permeabilized with cold-methanol at -20°C for 10 min and then stained with ARG62969 anti-Cytokeratin (pan) antibody [C-11].
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ARG62969 anti-Cytokeratin (pan) antibody [C-11] IHC-P image
Immunohistochemistry: Paraffin-embedded sections of guinea pig breast carcinoma stained with ARG62969 anti-Cytokeratin (pan) antibody [C-11].
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Review for anti-Cytokeratin (pan) antibody [C-11]
Application：WB
Sample：Mouse kidney, Rat kidney
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Clone References
Intracellular distribution of p300 and its differential recruitment to aggresomes in breast cancer.
Establishment and characterization of baboon embryonic stem cell lines: an Old World Primate model for regeneration and transplantation research.
ICC/IF / Baboon
