ARG66196
anti-Cytokeratin 8 antibody [SQab1718]
anti-Cytokeratin 8 antibody [SQab1718] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab1718] recognizes Cytokeratin 8 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab1718 |
| Target Name | Cytokeratin 8 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the C-terminus of Cytokeratin 8. |
| Conjugation | Un-conjugated |
| Alternate Names | Keratin, type II cytoskeletal 8; KO; CYK8; CK-8; Type-II keratin Kb8; K2C8; CARD2; Keratin-8; K8; CK8; Cytokeratin-8 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed using Tris/EDTA buffer (pH 9.0) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRT8 |
| Gene Full Name | keratin 8, type II |
| Background | This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012] |
| Function | Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. [UniProt] |
| Highlight | Related news: Cancer Pathology Markers (SQ clones) |
| Calculated MW | 54 kDa |
| PTM | Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. |
Images (2) Click the Picture to Zoom In
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ARG66196 anti-Cytokeratin 8 antibody [SQab1718] WB image
Western blot: 20 µg of HeLa cell lysate stained with ARG66196 anti-Cytokeratin 8 antibody [SQab1718] at 1:500 dilution, overnight at 4°C.
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ARG66196 anti-Cytokeratin 8 antibody [SQab1718] IHC-P image
Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human colon tissue stained with ARG66196 anti-Cytokeratin 8 antibody [SQab1718] at 1:200 dilution.
Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0
Customer's Feedback
Excellent
Review for anti-Cytokeratin 8 antibody [SQab1718]
Application：WB
Sample：HeLa
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
