ARG59499
anti-Cytokeratin 8 antibody
anti-Cytokeratin 8 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Cytokeratin 8 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Cytokeratin 8 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-483 of Human Cytokeratin 8 (NP_002264.1). |
| Conjugation | Un-conjugated |
| Alternate Names | Keratin, type II cytoskeletal 8; KO; CYK8; CK-8; Type-II keratin Kb8; K2C8; CARD2; Keratin-8; K8; CK8; Cytokeratin-8 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | HeLa and NIH/3T3 | ||||||||
| Observed Size | 53 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRT8 |
| Gene Full Name | keratin 8, type II |
| Background | This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012] |
| Function | Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix. [UniProt] |
| Calculated MW | 54 kDa |
| PTM | Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG59499 anti-Cytokeratin 8 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG59499 anti-Cytokeratin 8 antibody (green).
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ARG59499 anti-Cytokeratin 8 antibody WB image
Western blot: 25 µg of HeLa and NIH/3T3 cell lysates stained with ARG59499 anti-Cytokeratin 8 antibody at 1:1000 dilution.
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ARG59499 anti-Cytokeratin 8 antibody WB image
Western blot: 25 µg of extracts from normal (control) and KRT8 knockout (KO) HeLa cells, using ARG59499 anti-Cytokeratin 8 antibody at 1:1000 dilution.
