ARG66340
anti-Cytokeratin 7 antibody [SQab1888]
anti-Cytokeratin 7 antibody [SQab1888] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse
CK7/CK20 Carcinoma Study antibody
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab1888] recognizes Cytokeratin 7 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, ICC/IF, IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab1888 |
| Isotype | IgG |
| Target Name | Cytokeratin 7 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 1-100 of Cytokeratin 7. |
| Conjugation | Un-conjugated |
| Alternate Names | SCL; Sarcolectin; CK-7; Cytokeratin-7; CK7; Keratin, type II cytoskeletal 7; Keratin-7; K7; K2C7; Type-II keratin Kb7 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed using Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRT7 |
| Gene Full Name | keratin 7, type II |
| Background | Cytokeratin 7 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008] |
| Function | Cytokeratin 7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30318 CK7 / CK20 Carcinoma Antibody Duo Related products: Cytokeratin 7 antibodies; Cytokeratin 7 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones) More than a biomarker, CA19-9 is a therapeutic target of pancreatic cancer |
| Research Area | CK7/CK20 Carcinoma Study antibody |
| Calculated MW | 51 kDa |
| PTM | Arg-20 is dimethylated, probably to asymmetric dimethylarginine. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG66340 anti-Cytokeratin 7 antibody [SQab1888] IHC-P image
Immunohistochemistry: 10% Formalin-fixed and paraffin-embedded Mouse tumor tissue. Antigen Retrieval: Heat mediation was performed using Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66340 anti-Cytokeratin 7 antibody [SQab1888] at 1:3000 dilution for 30 min at 37ºC.
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ARG66340 anti-Cytokeratin 7 antibody [SQab1888] ICC/IF image
Immunofluorescence: HeLa cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66340 anti-Cytokeratin 7 antibody [SQab1888] (green) at 1:2,000 and 4°C. DAPI (blue) was used as the nuclear counter stain.
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ARG66340 anti-Cytokeratin 7 antibody [SQab1888] FACS image
Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66340 anti-Cytokeratin 7 antibody [SQab1888] (red) at 1:800 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
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ARG66340 anti-Cytokeratin 7 antibody [SQab1888] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human thyroid cancer tissue. Antigen Retrieval: Heat mediation was performed using Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66340 anti-Cytokeratin 7 antibody [SQab1888] at 1:3200 dilution.
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ARG66340 anti-Cytokeratin 7 antibody [SQab1888] WB image
Western blot: 10 µg of HeLa, A549 and HaCat cell lysates stained with ARG66340 anti-Cytokeratin 7 antibody [SQab1888] at 1:10000 dilution.
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ARG66340 anti-Cytokeratin 7 antibody [SQab1888] IP image
Immunoprecipitation: 0.4 mg of HeLa whole cell lysate immunoprecipitated (1:50) and stained with ARG66340 anti-Cytokeratin 7 antibody [SQab1888]. 1) ARG66340 IP in HeLa whole cell lysate, 2) PBS instead of ARG66340 in HeLa whole cell lysate, and 3) HeLa whole cell lysate, 10 µg (input).
Customer's Feedback
Excellent
Review for anti-Cytokeratin 7 antibody [SQab1888]
Application：IHC
Sample：Mouse tumor
Sample Preparation Method：Paraffin-embedded
Fixation Buffer：10% Formalin
Antigen Retrieval Buffer：Tris/EDTA buffer (pH 9.0)
Antigen Retrieval Temperature：98 ºC
Primary Antibody Dilution Factor：1:3000
Primary Antibody Incubation Time：30 min
Primary Antibody Incubation Temperature：37 ºC
Excellent
Review for anti-Cytokeratin 7 antibody [SQab1888]
Application：IHC
Sample：Mouse tumor
Sample Preparation Method：Paraffin-embedded
Fixation Buffer：10% Formalin
Antigen Retrieval Buffer：Tris/EDTA buffer (pH 9.0)
Antigen Retrieval Temperature：98 ºC
Primary Antibody Dilution Factor：1:3000
Primary Antibody Incubation Time：30 min
Primary Antibody Incubation Temperature：37 ºC
