ARG40395

anti-Cytokeratin 5 antibody

anti-Cytokeratin 5 antibody for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes Cytokeratin 5
Tested Reactivity Hu, Ms
Tested Application ICC/IF, IHC-Fr, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Cytokeratin 5
Antigen Species Human
Immunogen Synthetic peptide around the C-terminal region of Human Cytokeratin 5.
Conjugation Un-conjugated
Alternate Names DDD1; 58 kDa cytokeratin; Keratin, type II cytoskeletal 5; EBS2; Keratin-5; Cytokeratin-5; CK5; KRT5A; K5; CK-5; Type-II keratin Kb5; DDD

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-Fr1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 60 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 110308 Mouse KRT5

GeneID: 3852 Human KRT5

Swiss-port # P13647 Human Keratin, type II cytoskeletal 5

Swiss-port # Q922U2 Mouse Keratin, type II cytoskeletal 5

Gene Symbol KRT5
Gene Full Name keratin 5, type II
Background Cytokeratin 5 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
Calculated MW 62 kDa

Images (2) Click the Picture to Zoom In

  • ARG40395 anti-Cytokeratin 5 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human urithelial carcinoma stained with ARG40395 anti-Cytokeratin 5 antibody.

  • ARG40395 anti-Cytokeratin 5 antibody WB image

    Western blot: A431 cell lysate stained with ARG40395 anti-Cytokeratin 5 antibody.