ARG66324
anti-Cytokeratin 19 antibody [SQab1872]
anti-Cytokeratin 19 antibody [SQab1872] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab1872] recognizes Cytokeratin 19 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab1872 |
| Isotype | IgG |
| Target Name | Cytokeratin 19 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the N-terminus of Cytokeratin 19. |
| Conjugation | Un-conjugated |
| Alternate Names | Cytokeratin-19; K19; CK-19; K1CS; Keratin-19; CK19; Keratin, type I cytoskeletal 19 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRT19 |
| Gene Full Name | keratin 19, type I |
| Background | Cytokeratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008] |
| Function | Cytokeratin 19 involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. [UniProt] |
| Highlight | Related products: Cytokeratin 19 antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones) More than a biomarker, CA19-9 is a therapeutic target of pancreatic cancer Therapeutic strategies against PDAC |
| Calculated MW | 44 kDa |
Images (5) Click the Picture to Zoom In
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ARG66324 anti-Cytokeratin 19 antibody [SQab1872] ICC/IF image
Immunofluorescence: MCF-7 cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66324 anti-Cytokeratin 19 antibody [SQab1872] (green) at 1:40,000 and 4°C. DAPI (blue) was used as the nuclear counter stain.
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ARG66324 anti-Cytokeratin 19 antibody [SQab1872] FACS image
Flow Cytometry: MCF-7 cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66324 anti-Cytokeratin 19 antibody [SQab1872] (red) at 1:200 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control. .
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ARG66324 anti-Cytokeratin 19 antibody [SQab1872] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded thyroid cancer tissue stained with ARG66324 anti-Cytokeratin 19 antibody [SQab1872] at 1:40,000 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
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ARG66324 anti-Cytokeratin 19 antibody [SQab1872] WB image
Western blot: 10 µg of MCF7, Caco-2, HaCat and PC-3 cell lysates stained with ARG66324 anti-Cytokeratin 19 antibody [SQab1872] at 1:1000 dilution.
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ARG66324 anti-Cytokeratin 19 antibody [SQab1872] IP image
Immunoprecipitation: 0.4 mg of HaCat whole cell lysate immunoprecipitated (1:100) and stained with ARG66324 anti-Cytokeratin 19 antibody [SQab1872]. 1) ARG66324 IP in HaCat whole cell lysate, 2) PBS instead of ARG66324 in HaCat whole cell lysate, and 3) HaCat whole cell lysate, 10 µg (input).
