ARG66334
anti-Cytokeratin 18 antibody [SQab1882]
anti-Cytokeratin 18 antibody [SQab1882] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Rat
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab1882] recognizes Cytokeratin 18 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab1882 |
| Isotype | IgG |
| Target Name | Cytokeratin 18 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the C-terminus of Human Cytokeratin 18. |
| Conjugation | Un-conjugated |
| Alternate Names | Keratin, type I cytoskeletal 18; Cytokeratin-18; K18; CK-18; Cell proliferation-inducing gene 46 protein; Keratin-18; CYK18 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRT18 |
| Gene Full Name | keratin 18, type I |
| Background | Cytokeratin 18, together with its filament partner Cytokeratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Cytokeratin 18 involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. [UniProt] |
| Highlight | Related products: Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones) |
| Calculated MW | 48 kDa |
| PTM | Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG66334 anti-Cytokeratin 18 antibody [SQab1882] ICC/IF image
Immunofluorescence: HCT-116 cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66334 anti-Cytokeratin 18 antibody [SQab1882] (green) at 1:2,000 and 4°C. DAPI (blue) was used as the nuclear counter stain.
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ARG66334 anti-Cytokeratin 18 antibody [SQab1882] FACS image
Flow Cytometry: HCT-116 cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66334 anti-Cytokeratin 18 antibody [SQab1882] (red) at 1:2,000 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control. .
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ARG66334 anti-Cytokeratin 18 antibody [SQab1882] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded colonic adenocarcinoma tissue stained with ARG66334 anti-Cytokeratin 18 antibody [SQab1882] at 1:1,600 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
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ARG66334 anti-Cytokeratin 18 antibody [SQab1882] WB image
Western blot: 10 µg of HeLa, A431, HepG2, T47D, MCF7 and A549 cell lysates stained with ARG66334 anti-Cytokeratin 18 antibody [SQab1882] at 1:10000 dilution.
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ARG66334 anti-Cytokeratin 18 antibody [SQab1882] IP image
Immunoprecipitation: 0.4 mg of HeLa whole cell lysate immunoprecipitated (1:25) and stained with ARG66334 anti-Cytokeratin 18 antibody [SQab1882]. 1) ARG66334 IP in HeLa whole cell lysate, 2) PBS instead of ARG66334 in HeLa whole cell lysate, and 3) HeLa whole cell lysate, 10 µg (input).
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ARG66334 anti-Cytokeratin 18 antibody [SQab1882] WB image
Western blot: 10 µg of Rat liver lysate stained with ARG66334 anti-Cytokeratin 18 antibody [SQab1882] at 1:5000 dilution.
