ARG66352
anti-Cytokeratin 18 antibody [SQab18102]
anti-Cytokeratin 18 antibody [SQab18102] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab18102] recognizes Cytokeratin 18 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab18102 |
| Isotype | IgG |
| Target Name | Cytokeratin 18 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide within aa. 330-430 of Human Cytokeratin 18. |
| Conjugation | Un-conjugated |
| Alternate Names | Keratin, type I cytoskeletal 18; Cytokeratin-18; K18; CK-18; Cell proliferation-inducing gene 46 protein; Keratin-18; CYK18 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT for 30 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | HCT116; Human liver tissue | ||||||
| Observed Size | ~ 48 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRT18 |
| Gene Full Name | keratin 18, type I |
| Background | Cytokeratin 18, together with its filament partner Cytokeratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Cytokeratin 18 involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. [UniProt] |
| Highlight | Related products: Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones) |
| Calculated MW | 48 kDa |
| PTM | Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG66352 anti-Cytokeratin 18 antibody [SQab18102] WB image (Customer's Feedback)
Western blot: HCT116 cell lysate stained with ARG66352 anti-Cytokeratin 18 antibody [SQab18102] at 1:1000 dilution.
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ARG66352 anti-Cytokeratin 18 antibody [SQab18102] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded liver tissue stained with ARG66352 anti-Cytokeratin 18 antibody [SQab18102]. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).
Customer's Feedback
Excellent
Review for anti-Cytokeratin 18 antibody [SQab18102]
Application：WB
Sample：HCT116
Sample Loading Amount：20 μg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：Overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：HRP
