ARG62975
anti-Cytokeratin 18 antibody [C-04]
anti-Cytokeratin 18 antibody [C-04] for ELISA,Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Cat,Cow,Dog,Goat,Hamster,Horse,Mammal,Pig,Sheep
Controls and Markers antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [C-04] recognizes Cytokeratin 18 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Cat, Cow, Dog, Goat, Hm, Hrs, Mamm, Pig, Sheep |
| Tested Application | ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Specificity | The clone C-04 reacts with Cytokeratin 18 (45 kDa), a member of intermediate filaments subfamily represented in epithelial tissues. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | C-04 |
| Isotype | IgG1 |
| Target Name | Cytokeratin 18 |
| Immunogen | Cytoskeleton preparation of epidermal carcinoma cell line A431. |
| Conjugation | Un-conjugated |
| Alternate Names | Keratin, type I cytoskeletal 18; Cytokeratin-18; K18; CK-18; Cell proliferation-inducing gene 46 protein; Keratin-18; CYK18 |
Application Instructions
| Application Suggestion |
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| Application Note | Western Blotting: incubate overnight in 4°C. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (10% separating gel). ELISA: Application note: The antibody C-04 has been tested as the capture antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with antibody DA-7 (cat. no. ARG62977) and with the antibody DC-10 (cat. no. ARG53186). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | WB: HeLa and MCF-7 IHC-P: Colon and kidney tissue. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from hybridoma culture supernatant by protein A-affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | KRT18 |
| Gene Full Name | keratin 18, type I |
| Background | Cytokeratin 18, together with its filament partner Cytokeratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Cytokeratin 18 involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. [UniProt] |
| Research Area | Controls and Markers antibody; Signaling Transduction antibody |
| Calculated MW | 48 kDa |
| PTM | Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation. |
Images (4) Click the Picture to Zoom In
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ARG62975 anti-Cytokeratin 18 antibody [C-04] IHC-P image
Immunohistochemistry: Human kidney (paraffin-embedded sections) stained with ARG62975 anti-Cytokeratin 18 antibody [C-04].
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ARG62975 anti-Cytokeratin 18 antibody [C-04] IHC-P image
Immunohistochemistry: Human colon (paraffin-embedded sections) stained with ARG62975 anti-Cytokeratin 18 antibody [C-04].
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ARG62975 anti-Cytokeratin 18 antibody [C-04] FACS image
Flow Cytometry: Separation of stained HeLa cells (red-filled) from unstained HeLa cells (black-dashed). Cells were stained with ARG62975 anti-Cytokeratin 18 antibody [C-04] at 0.6 µg/ml dilution, followed by FITC-conjugated Goat anti-Mouse antibody.
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ARG62975 anti-Cytokeratin 18 antibody [C-04] FACS image
Flow Cytometry: HeLa cell suspension stained with ARG62975 anti-Cytokeratin 18 antibody [C-04] at 0.6 µg/ml dilution, followed by FITC-conjugated Goat anti-Mouse antibody.
Clone References
TP53 supports basal-like differentiation of mammary epithelial cells by preventing translocation of deltaNp63 into nucleoli.
ICC/IF / Human
Development of hepatocellular carcinoma in a murine model of nonalcoholic steatohepatitis induced by use of a high-fat/fructose diet and sedentary lifestyle.
IHC / Mouse
The miR-424(322)/503 cluster orchestrates remodeling of the epithelium in the involuting mammary gland.
IHC-P / Mouse
Miz1 deficiency in the mammary gland causes a lactation defect by attenuated Stat5 expression and phosphorylation.
WB / Mouse
Salisphere derived c-Kit+ cell transplantation restores tissue homeostasis in irradiated salivary gland.
IHC-Fr / Mouse
Feasibility of fast dynamic MRI for noninvasive monitoring during ectopic liver cell transplantation to the spleen in a porcine model.
IHC / Pig
Conditional activation of Pik3ca(H1047R) in a knock-in mouse model promotes mammary tumorigenesis and emergence of mutations.
ICC/IF / Mouse
Stemness markers characterize IGR-CaP1, a new cell line derived from primary epithelial prostate cancer.
FACS / Human
Suppression of keratin 18 gene expression in bovine blastocysts by RNA interference.
ICC/IF / Cow
Generation of a prostate from a single adult stem cell.
FACS, IHC-Fr / Mouse
