ARG55964
anti-Cytokeratin 14 antibody [LL002]
anti-Cytokeratin 14 antibody [LL002] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Dog,Elephant,Pig
Overview
| Product Description | Mouse Monoclonal antibody [LL002] recognizes Cytokeratin 14 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Dog, Elp, Pig |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | LL002 |
| Isotype | IgG3 |
| Target Name | Cytokeratin 14 |
| Antigen Species | Human |
| Immunogen | A 15-mer synthetic peptide from the C-terminus of Human Cytokeratin 14. |
| Conjugation | Un-conjugated |
| Alternate Names | K14; CK-14; Cytokeratin-14; Keratin, type I cytoskeletal 14; EBS3; EBS4; NFJ; Keratin-14; CK14 |
Application Instructions
| Application Suggestion |
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| Application Note | Antigen retrieval for IHC-P: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 53 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRT14 |
| Gene Full Name | keratin 14, type I |
| Background | This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008] |
| Function | The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. [UniProt] |
| Cellular Localization | Cytoplasmic |
| Calculated MW | 52 kDa |
| PTM | A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus. Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex. |
Images (5) Click the Picture to Zoom In
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ARG55964 anti-Cytokeratin 14 antibody [LL002] IHC-P image
Immunohistochemistry: Human prostate (10X) stained with ARG55964 anti-Cytokeratin 14 antibody [LL002].
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ARG55964 anti-Cytokeratin 14 antibody [LL002] WB image
Western blot: A431 cell lysate (epidermoid carcinoma) stained with ARG55964 anti-Cytokeratin 14 antibody [LL002] at 1 µg/ml dilution.
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ARG55964 anti-Cytokeratin 14 antibody [LL002] IHC-P image
Immunohistochemistry: Human prostate (20X) stained with ARG55964 anti-Cytokeratin 14 antibody [LL002].
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ARG55964 anti-Cytokeratin 14 antibody [LL002] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human prostate stained with ARG55964 anti-Cytokeratin 14 antibody [LL002].
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ARG55964 anti-Cytokeratin 14 antibody [LL002] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human prostate stained with ARG55964 anti-Cytokeratin 14 antibody [LL002].
