ARG54065
anti-Cyclin H antibody
anti-Cyclin H antibody for Immunoprecipitation,Western blot and Human
Cell Biology and Cellular Response antibody; Gene Regulation antibody
Overview
| Product Description | Mouse Monoclonal antibody recognizes CCNH |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Target Name | Cyclin H |
| Antigen Species | Human |
| Immunogen | Purified recombinant human Cyclin H protein fragments expressed in E.coli. |
| Conjugation | Un-conjugated |
| Alternate Names | MO15-associated protein; CAK; CycH; Cyclin-H; p37; p34 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | 38 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol |
| Preservative | 0.2% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CCNH |
| Gene Full Name | cyclin H |
| Background | Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle. |
| Function | Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle. [UniProt] |
| Cellular Localization | Nucleus. |
| Research Area | Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| Calculated MW | 38 kDa |
Images (2) Click the Picture to Zoom In
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ARG54065 anti-Cyclin H antibody WB image
Western blot: A431 cell lysate stained with ARG54065 anti-Cyclin H antibody at 1:1000 dilution.
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ARG54065 anti-Cyclin H antibody IP image
Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG54065 anti-Cyclin H antibody.
