ARG52923
anti-Cyclin D1 antibody [SP4]
anti-Cyclin D1 antibody [SP4] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody
Overview
Product Description | Rabbit Monoclonal antibody [SP4] recognizes Cyclin D1 |
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Tested Reactivity | Hu, Ms, Rat |
Predict Reactivity | Dog |
Tested Application | ICC/IF, IHC-P, WB |
Host | Rabbit |
Clonality | Monoclonal |
Clone | SP4 |
Isotype | IgG |
Target Name | Cyclin D1 |
Antigen Species | Human |
Immunogen | Synthetic peptide from C-terminus of human cyclin D1. |
Conjugation | Un-conjugated |
Alternate Names | B-cell lymphoma 1 protein; PRAD1; U21B31; D11S287E; BCL-1; G1/S-specific cyclin-D1; BCL-1 oncogene; BCL1; PRAD1 oncogene |
Application Instructions
Application Suggestion |
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Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 30 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Positive Control | Mantle Cell Lymphoma, Tonsil, MCF-7 |
Properties
Form | Liquid |
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Buffer | Tissue culture supernatant in TBS (pH 7.5), 1% BSA and < 0.1% Sodium azide |
Preservative | < 0.1% Sodium azide |
Stabilizer | 1% BSA |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Background | Cyclin D1 or PRAD-1 or bcl-1 is one of the key cell cycle regulators, and functions in association with cdk4 and / or cdk6 by phosphorylating the Rb protein. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL). |
Cellular Localization | Nucleus |
Highlight | Related products: Cyclin D1 antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Lymphoma |
Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody |
Calculated MW | 34 kDa |
PTM | Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex. Ubiquitinated, primarily as 'Lys-48'-linked polyubiquitination. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB. Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. SCF-type ubiquitination is dependent on Thr-286 phosphorylation (By similarity). Ubiquitinated also by UHRF2 apparently in a phosphorylation-independent manner. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2; leading to its stabilization. |
Images (13) Click the Picture to Zoom In
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ARG52923 anti-Cyclin D1 antibody [SP4] WB image
Western blot: 30 µg of HeLa, SW480, 3T3, and Mouse kidney lysates stained with ARG52923 anti-Cyclin D1 antibody [SP4] at 1:500 dilution.
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ARG52923 anti-Cyclin D1 antibody [SP4] ICC/IF image
Immunofluorescence: H1793 cells were fixed with cold methanol:acetone (1:1) for 5 minutes at -20°C, permeabilized with 0.3% Triton X-100 for 30 min at RT then blocked with 1% goat serum for 30 min at RT. Cells were stained with ARG52923 anti-Cyclin D1 antibody [SP4] (red) at 1:100 dilution for 1 hour at RT and DAPI (blue) was used as the nuclear counter stain.
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ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image
Immunohistochemistry: Human B Cell Lymphoma stained with Cyclin D1 antibody [SP4] (ARG52923)
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ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image
Immunohistochemistry: Human Endometrial Adenocarcinoma stained with Cyclin D1 antibody [SP4] (ARG52923)
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ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image
Immunohistochemistry: Human HK Lymphoma stained with Cyclin D1 antibody [SP4] (ARG52923)
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ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image
Immunohistochemistry: Human Lung Adenocarcinoma stained with Cyclin D1 antibody [SP4] (ARG52923)
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ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image
Immunohistochemistry: Human Mantle Cell Lymphoma stained with Cyclin D1 antibody [SP4] (ARG52923)
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ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image
Immunohistochemistry: Human Ovarian Adenocarcinoma stained with Cyclin D1 antibody [SP4] (ARG52923)
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ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image
Immunohistochemistry: Human Pancreatic Adenocarcinoma stained with Cyclin D1 antibody [SP4] (ARG52923)
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ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image
Immunohistochemistry: Human Prostate Adenocarcinoma stained with Cyclin D1 antibody [SP4] (ARG52923)
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ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image
Immunohistochemistry: Human Renal Cell Carcinoma stained with Cyclin D1 antibody [SP4] (ARG52923)
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ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image
Immunohistochemistry: Human Tonsil stained with Cyclin D1 antibody [SP4] (ARG52923)
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ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image
Immunohistochemistry: Human Mantle Cell Lymphoma stained with Cyclin D1 antibody [SP4] (ARG52923)
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