ARG43683
anti-Cyclin A2 antibody
anti-Cyclin A2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Cyclin A2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Cyclin A2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to Human Cyclin A2. |
| Conjugation | Un-conjugated |
| Protein Full Name | Cyclin-A2 |
| Alternate Names | Cyclin-A2; Cyclin-A; CCN1; CCNA |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | ~50 - 55 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | 50 mM Tris-Glycine (pH 7.4), 150 mM NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Concentration | Batch dependent |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CCNA2 |
| Gene Full Name | cyclin A2 |
| Background | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. [provided by RefSeq, Jul 2008] |
| Function | Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions. [UniProt] |
| Cellular Localization | Cytoplasm; Nucleus |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| Calculated MW | 49 kDa |
| PTM | Polyubiquitinated via 'Lys-11'-linked ubiquitin by the anaphase-promoting complex (APC/C), leading to its degradation by the proteasome. Deubiquitinated and stabilized by USP37 enables entry into S phase. |
Images (2) Click the Picture to Zoom In
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ARG43683 anti-Cyclin A2 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsi stained with ARG43683 anti-Cyclin A2 antibody at 1:100 dilution. Antigen Retrieval: Heat mediated was performed using sodium citrate buffer (pH 6.0).
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ARG43683 anti-Cyclin A2 antibody WB image
Western blot: C6, 3T3 and Hela cells lysate stained with ARG43683 anti-Cyclin A2 antibody at 1:1000 dilution.
