ARG20786
anti-Collagen III antibody, pre-adsorbed
anti-Collagen III antibody, pre-adsorbed for ELISA,Electron microscopy,Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Bovine,Chicken,Dog,Hamster,Pig,Rabbit
Publication8
Overview
| Product Description | Goat Polyclonal antibody recognizes Collagen III |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov, Chk, Dog, Hm, Pig, Rb |
| Tested Application | ELISA, EM, FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| Specificity | The antibody reacts with conformational determinants on type III collagen. The antibody is pre-adsorbed with Collagen types I, II, IV, V and VI, so the antibody may not react with Collagen types I, II, IV, V and VI. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Collagen III |
| Antigen Species | Human |
| Immunogen | Human Type III collagen |
| Conjugation | Un-conjugated |
| Alternate Names | EDS4A; Collagen alpha-1(III) chain |
Application Instructions
| Pre Adsorbed | Collagen types I, II, IV, V and VI. | ||||||||||||||||
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| Application Suggestion |
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| Application Note | WB: The antibody reacts with native and denature form of Collagen III protein. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | BBS (pH 8.2) |
| Concentration | 0.4 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | COL3A1 |
| Gene Full Name | collagen, type III, alpha 1 |
| Background | This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008] |
| Function | Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of GPR56 in the developing brain and binding to GPR56 inhibits neuronal migration and activates the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12. [UniProt] |
| Calculated MW | 139 kDa |
| PTM | Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. |
Images (4) Click the Picture to Zoom In
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ARG20786 anti-Collagen III antibody, pre-adsorbed ICC/IF image
Immunofluorescence: Rabbit bone marrow stromal cells stained with ARG20786 anti-Collagen III antibody, pre-adsorbed.
From Cheng-Chang Lu et al. Bone Joint Res. (2023), doi: 10.1302/2046-3758.121.BJR-2021-0434.R2, Fig. 6.
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ARG20786 anti-Collagen III antibody (pre-adsorbed) IHC-P image
Immunohistochemistry: Paraffin-embedded Human gastric cancer tissue stained with ARG23772 Goat IgG Isotype Control antibody (left) and ARG20786 anti-Collagen III antibody (pre-adsorbed) (right) followed by ARG23857 Swine anti-Goat IgG (H+L) antibody (HRP) (pre-adsorbed), DAB and hematoxylin.
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ARG20786 anti-Collagen III antibody, pre-adsorbed WB image
Western blot: Embryonic chick cardiac tissue samples separated by SDS-PAGE and stained with ARG20786 anti-Collagen III antibody (pre-adsorbed) under reducing conditions.
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ARG20786 anti-Collagen III antibody (pre-adsorbed) IHC-P image
Immunohistochemistry: Paraffin-embedded Human gastric cancer tissue stained with ARG23772 Goat IgG Isotype Control antibody (left) and ARG20786 anti-Collagen III antibody (pre-adsorbed) (right) followed by ARG23857 Swine anti-Goat IgG (H+L) antibody (HRP) (pre-adsorbed), DAB and hematoxylin.
Specific References
Bone marrow stromal and anterior cruciate ligament remnant cell co-culture-derived extracellular vesicles promote cell activity in both cell types
ICC/IF / Rabbit
Effect of lyophilized exosomes derived from umbilical cord stem cells on chronic anterior cruciate ligament cell injury
ICC/IF / Human
Anterior cruciate ligament remnant preservation attenuates apoptosis and enhances the regeneration of hamstring tendon graft
ICC/IF / Rabbit
Vinpocetine Ameliorates Metabolic-Syndrome-Associated Bladder Overactivity in Fructose-Fed Rats by Restoring Succinate-Modulated cAMP Levels and Exerting Anti-Inflammatory Effects in the Bladder Detrusor Muscle
WB / Mouse
Comparative therapeutic strategies for preventing aortic rupture in a mouse model of vascular Ehlers-Danlos syndrome
IHC-P / Mouse
Quantitative proteomic comparison of myofibroblasts derived from bone marrow and cornea.
WB / Rabbit
Extracorporeal shock wave promotes activation of anterior cruciate ligament remnant cells and their paracrine regulation of bone marrow stromal cells' proliferation, migration, collagen synthesis, and differentiation.
ICC/IF / Human
Ba-Wei-Die-Huang-Wan (Hachimi-jio-gan) can ameliorate ketamine-induced cystitis by modulating neuroreceptors, inflammatory mediators, and fibrogenesis in a rat model.
WB / Rat
