ARG20787
anti-Collagen II antibody, pre-adsorbed
anti-Collagen II antibody, pre-adsorbed for ELISA,Electron microscopy,FLISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Bovine,Chicken,Pig,Rabbit,Sheep
Publication13
Overview
| Product Description | Goat Polyclonal antibody recognizes Collagen II |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov, Chk, Pig, Rb, Sheep |
| Tested Application | ELISA, EM, FLISA, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Specificity | The antibody reacts with conformational determinants on type II collagen. The antibody is pre-adsorbed with Collagen types I, III, IV, V and VI, so the antibody may not react with Collagen types I, III, IV, V and VI. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Collagen II |
| Antigen Species | Human |
| Immunogen | Collagen II |
| Conjugation | Un-conjugated |
| Alternate Names | AOM; ANFH; SEDC; STL1; COL11A3; Collagen alpha-1(II) chain; Alpha-1 type II collagen) |
Application Instructions
| Pre Adsorbed | Collagen types I, III, IV, V and VI. | ||||||||||||||||||
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| Application Suggestion |
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| Application Note | WB: The antibody reacts with native and denature form of Collagen II protein. IHC-P: Antigen Retrieval: treat sections with 2 µg/ml proteinase K at 37°C for 15 min before staining. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | BBS (pH 8.2) |
| Concentration | 0.4 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | COL2A1 |
| Gene Full Name | collagen, type II, alpha 1 |
| Background | This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008] |
| Function | Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. [UniProt] |
| Calculated MW | 142 kDa |
| PTM | Probably 3-hydroxylated on prolines by LEPREL1 (By similarity). Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains. The N-telopeptide is covalently linked to the helical COL2 region of alpha 1(IX), alpha 2(IX) and alpha 3(IX) chain. The C-telopeptide is covalently linked to an another site in the helical region of alpha 3(IX) COL2. |
Images (4) Click the Picture to Zoom In
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ARG20787 anti-Collagen II antibody, pre-adsorbed ICC/IF image
Immunofluorescence: Porcine primary chondrocytes fixed with 4% Paraformaldehyde for 10 min at RT and blocked by 1% BSA, 0.1% Triton for 30 min at RT. Cells were incubated in 5% BSA and stained with ARG20787 anti-Collagen II antibody (pre-adsorbed) at 1:100 for 3 hours at RT.
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ARG20787 anti-Collagen II antibody (pre-adsorbed) IHC-Fr image
Immunohistochemistry: Frozen section of newborn Mouse rib tissue stained with ARG20787 anti-Collagen II antibody (pre-adsorbed) followed by Rabbit anti-Goat IgG (H+L) antibody (FITC) and DAPI.
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ARG20787 anti-Collagen II antibody, pre-adsorbed IHC-P image
Immunohistochemistry: Paraffin-embedded tibiae section from E15 mouse was stained with ARG20787 anti-Collagen II antibody (pre-adsorbed).
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ARG20787 anti-Collagen II antibody (pre-adsorbed) IHC-Fr image
Immunohistochemistry: Frozen section of newborn Mouse cartilage tissue stained with ARG20787 anti-Collagen II antibody (pre-adsorbed) (right) followed by Donkey anti-Goat IgG (H+L) antibody (Alexa Fluor® 555) and DAPI.
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Application：IF/ICC
Sample：Porcine primary chondrocytes
Fixation Buffer：4% Paraformaldehyde
Fixation Time：10 min
Fixation Temperature：RT ºC
Primary Antibody Dilution Factor：1:100
Primary Antibody Incubation Time：3 hours
Primary Antibody Incubation Temperature：RT ºC
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