ARG40700
anti-Collagen I antibody
anti-Collagen I antibody for Immunocytochemistry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Collagen I |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Hm |
| Tested Application | ICC, IHC-Fr, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Collagen I |
| Antigen Species | Mouse |
| Immunogen | Synthetic peptide corresponding to aa. 1192-1207 of Mouse Collagen I. (QPPQEKSQDGGRYYRA) |
| Conjugation | Un-conjugated |
| Alternate Names | OI1; OI2; OI3; OI4; EDSC; Collagen alpha-1(I) chain; Alpha-1 type I collagen |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Thimerosal and 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | COL1A1 |
| Gene Full Name | collagen, type I, alpha 1 |
| Background | This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008] |
| Function | Type I collagen is a member of group I collagen (fibrillar forming collagen). [UniProt] |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. [UniProt] |
| Highlight | Related products: Collagen I antibodies; Collagen I ELISA Kits; Collagen I Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: New antibody panels for Myofibroblasts and CAFs |
| Calculated MW | 139 kDa |
| PTM | Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains. O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. [UniProt] |
Images (8) Click the Picture to Zoom In
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ARG40700 anti-Collagen I antibody ICC image
Immunocytochemistry: NIH/3T3 cells were blocked with 10% goat serum and then stained with ARG40700 anti-Collagen I antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG40700 anti-Collagen I antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat lung tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40700 anti-Collagen I antibody at 1 µg/ml, overnight at 4°C.
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ARG40700 anti-Collagen I antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40700 anti-Collagen I antibody at 1 µg/ml, overnight at 4°C.
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ARG40700 anti-Collagen I antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse lung tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40700 anti-Collagen I antibody at 2 µg/ml dilution, overnight at 4°C.
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ARG40700 anti-Collagen I antibody IHC-Fr image
Immunohistochemistry: Frozen section of Human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then stained with 1 μg/ml dilution, overnight at 4°C.
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ARG40700 anti-Collagen I antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40700 anti-Collagen I antibody (red) at 1 µg/ml, overnight at 4°C.
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ARG40700 anti-Collagen I antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40700 anti-Collagen I antibody (red) at 1 µg/ml, overnight at 4°C.
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ARG40700 anti-Collagen I antibody WB image
Western blot: 50 µg of samples under reducing conditions. Rat lung, Human placenta and Rat testis tissue lysates stained with ARG40700 anti-Collagen I antibody at 0.5 µg/ml, overnight at 4°C.
