ARG52247
anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody
anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Signaling Transduction antibody
Publication3
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Collagen I, alpha 1 propeptide (Procollagen I) |
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| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Amph, Bird, Fsh, Mamm |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | This antibody recognizes pro-collagen I, but not mature form of collagen I. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Pro-Collagen I |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to amino acid residues specific to the collagen I, alpha 1 propeptide. |
| Conjugation | Un-conjugated |
| Alternate Names | OI1; OI2; OI3; OI4; EDSC; Collagen alpha-1(I) chain; Alpha-1 type I collagen |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the propeptide portion of the ~180 kDa collagen I α1 polypeptide in human lung fibroblast extract. The antibody also works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90º C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, the antibody recognizes collagen I molecules that are still associated with the cells in which they were synthesized. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Human lung; Mouse lung; Rat lung | ||||||||
| Observed Size | 180- 210 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | PBS |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | COL1A1 |
| Gene Full Name | collagen, type I, alpha 1 |
| Background | Collagen is an extracellular matrix protein that serves as a scaffold defining the shape and mechanical properties of many tissues and organs including skin, tendon, artery walls, fibrocartilage, bone and teeth. Type 1 collagen is the must abundant protein in mammals. Collagens are synthesized with N-terminal and C-terminal propeptides that are cleaved during maturation and secretion. After cleavage of the propeptides, the most N-terminal and C-terminal remaining sequences are known as telopeptides. Mutations in the collagen 1, alpha 1 gene (COL1A1) are known to cause osteogenesis imperfecta (aka brittle bone disease) (Byers 1989). Furthermore, mutations found in the fist 90 residues of the helical region of alpha 1 collagen have been implicated in the prevention or delayed removal of the procollagen N-propeptide leading to a combined osteogenesis imperfecta and Ehlers-Danlos syndrome (EDS) phenotype (Cabral et al., 2005). |
| Highlight | Related products: Collagen I antibodies; Collagen I ELISA Kits; Collagen I Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: New antibody panels for Myofibroblasts and CAFs |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 139 kDa |
| PTM | Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains. O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. |
Images (3) Click the Picture to Zoom In
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ARG52247 anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody ICC/IF image
Immunofluorescence: Rat bone marrow mesenchymal stem cell stained with ARG52247 anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody.
From Jiahao Chen et al. IOPscience (2023), doi: 10.1088/1748-605X/ad0476, Fig. 4f.
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ARG52247 anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody WB image
Western blot: 30 µg of Rat lung lysate stained with ARG52247 anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody at 1:1000 dilution.
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ARG52247 anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody IHC image
Immunohistochemistry: Fibrotic Mouse lung tissue stained with ARG52247 anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody showing specific staining of collagen I molecules (red) that are still associated with the cells in which they were synthesized.
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Review for anti-Collagen I, alpha 1 propeptide (Procollagen I) antibody
Application：WB
Sample：Rat lung
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
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