ARG63780
anti-Caveolin 1 antibody
anti-Caveolin 1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Endosome Marker antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Caveolin 1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Dog, Pig |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Specificity | No cross-reactivity expected to Caveolin 2 and Caveolin 3 |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Caveolin 1 |
| Antigen Species | Human |
| Immunogen | C-DELSEKQVYDAH |
| Conjugation | Un-conjugated |
| Alternate Names | CGL3; LCCNS; PPH3; MSTP085; VIP21; BSCL3; Caveolin-1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.[provided by RefSeq, Mar 2010] |
| Research Area | Endosome Marker antibody |
| Calculated MW | 20 kDa |
| PTM | The initiator methionine for isoform 2 is removed during or just after translation. The new N-terminal amino acid is then N-acetylated. Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress. |
Images (7) Click the Picture to Zoom In
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ARG63780 anti-Caveolin 1 antibody WB image
Western Blot: Human Spleen lysate (35 µg protein in RIPA buffer) stained with ARG63780 anti-Caveolin 1 antibody at 0.01 µg/ml dilution.
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ARG63780 anti-Caveolin 1 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed A431 cells permeabilized with 0.15% Triton. Cells were stained with ARG63780 anti-Caveolin 1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63780 anti-Caveolin 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human heart tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63780 anti-Caveolin 1 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63780 anti-Caveolin 1 antibody WB image
Western blot: 35 µg of A431 (A) and A549 (B) cell lysates (in RIPA buffer) stained with ARG63780 anti-Caveolin 1 antibody at 0.003 µg/ml (A) and 0.001 µg/ml (B) dilutions and incubated at RT for 1 hour.
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ARG63780 anti-Caveolin 1 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG63780 anti-Caveolin 1 antibody (blue line) at 10 µg/ml dilution and overnight, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
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ARG63780 anti-Caveolin 1 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG63780 anti-Caveolin 1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63780 anti-Caveolin 1 antibody WB image
Western blot: 35 µg of Human heart lysate (in RIPA buffer) stained with ARG63780 anti-Caveolin 1 antibody at 0.003 µg/ml dilution and incubated at RT for 1 hour.
