ARG66328
anti-Caspase 3 antibody [SQab1876]
anti-Caspase 3 antibody [SQab1876] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab1876] recognizes Caspase 3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IP, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab1876 |
| Isotype | IgG |
| Target Name | Caspase 3 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 1-100 of Human Caspase 3. |
| Conjugation | Un-conjugated |
| Alternate Names | CASP3; Caspase 3; Apopain; CPP32; CPP32B; Caspase 3, Apoptosis-Related Cysteine Peptidase; Caspase 3, Apoptosis-Related Cysteine Protease; SREBP Cleavage Activity 1; Cysteine Protease CPP32; Protein Yama; EC 3.4.22.56; Caspase-3; CASP-3; CPP-32; SCA-1; Yama; PARP Cleavage Protease; Procaspase3; EC 3.4.22 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CASP3 |
| Gene Full Name | caspase 3, apoptosis-related cysteine peptidase |
| Background | The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017] |
| Function | Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30105 Apoptosis Marker Antibody Duo (Caspase3, PARP) ARG30110 Mitochondria/Caspase dependant Apoptosis Antibody Panel (Caspase3, Caspase9, Cytochrome c, PARP) (WB) Related products: Caspase 3 antibodies; Caspase 3 Duos / Panels; |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody |
| Calculated MW | 32 kDa, 17 kDa (cleaved) |
| PTM | Acetylation, Phosphoprotein, S-nitrosylation, Zymogen. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG66328 anti-Caspase 3 antibody [SQab1876] ICC/IF image
Immunofluorescence: Jurkat cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66328 anti-Caspase 3 antibody [SQab1876] (red) at 1:800 and 4°C. DAPI (blue) was used as the nuclear counter stain.
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ARG66328 anti-Caspase 3 antibody [SQab1876] FACS image
Flow Cytometry: Ramos cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66328 anti-Caspase 3 antibody [SQab1876] (red) at 1:1,000 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control. .
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ARG66328 anti-Caspase 3 antibody [SQab1876] WB image
Western blot: 20 µg of Jurkat treated with 1 µM staurosporine for 4h (+) or untreated (-). The blots were stained with ARG66328 anti-Caspase 3 antibody [SQab1876] at 1:2000 dilution.
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ARG66328 anti-Caspase 3 antibody [SQab1876] IP image
Immunoprecipitation: 0.4 mg of Jurkat whole cell lysate immunoprecipitated (1:50) and stained with ARG66328 anti-Caspase 3 antibody [SQab1876]. 1) ARG66328 IP in Jurkat whole cell lysate, 2) PBS instead of ARG66328 in Jurkat whole cell lysate, and 3) Jurkat whole cell lysate, 10 µg (input).
