anti-Caspase 3 antibody [SQab1876]

anti-Caspase 3 antibody [SQab1876] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody


Product Description Recombinant Rabbit Monoclonal antibody [SQab1876] recognizes Caspase 3
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IP, WB
Host Rabbit
Clonality Monoclonal
Clone SQab1876
Isotype IgG
Target Name Caspase 3
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 1-100 of Human Caspase 3.
Conjugation Un-conjugated
Alternate Names CPP-32; Caspase-3; EC; Apopain; CASP-3; CPP32; Cysteine protease CPP32; SCA-1; SREBP cleavage activity 1; CPP32B; Protein Yama

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:200 - 1:1000
ICC/IF1:200 - 1:800
WB1:1000 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 836 Human CASP3

Swiss-port # P42574 Human Caspase-3

Gene Symbol CASP3
Gene Full Name caspase 3, apoptosis-related cysteine peptidase
Background This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]
Function Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
Calculated MW 32 kDa, 17 kDa (cleaved)
PTM Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.

S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. [UniProt]

Images (4) Click the Picture to Zoom In

  • ARG66328 anti-Caspase 3 antibody [SQab1876] ICC/IF image

    Immunofluorescence: Jurkat cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66328 anti-Caspase 3 antibody [SQab1876] (red) at 1:800 and 4°C. DAPI (blue) was used as the nuclear counter stain.

  • ARG66328 anti-Caspase 3 antibody [SQab1876] FACS image

    Flow Cytometry: Ramos cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66328 anti-Caspase 3 antibody [SQab1876] (red) at 1:1,000 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control. .

  • ARG66328 anti-Caspase 3 antibody [SQab1876] WB image

    Western blot: 20 µg of Jurkat treated with 1 µM staurosporine for 4h (+) or untreated (-). The blots were stained with ARG66328 anti-Caspase 3 antibody [SQab1876] at 1:2000 dilution.

  • ARG66328 anti-Caspase 3 antibody [SQab1876] IP image

    Immunoprecipitation: 0.4 mg of Jurkat whole cell lysate immunoprecipitated (1:50) and stained with ARG66328 anti-Caspase 3 antibody [SQab1876]. 1) ARG66328 IP in Jurkat whole cell lysate, 2) PBS instead of ARG66328 in Jurkat whole cell lysate, and 3) Jurkat whole cell lysate, 10 µg (input).