ARG54938

anti-Caspase 3 antibody

anti-Caspase 3 antibody for ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
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publication_link Publication6

Overview

Product Description Rabbit Polyclonal antibody recognizes Caspase 3
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Caspase 3
Antigen Species Human
Immunogen Recombinant protein of Human Caspase-3 (NP_004337.2)
Conjugation Un-conjugated
Alternate Names CASP3; Caspase 3; Apopain; CPP32; CPP32B; Caspase 3, Apoptosis-Related Cysteine Peptidase; Caspase 3, Apoptosis-Related Cysteine Protease; SREBP Cleavage Activity 1; Cysteine Protease CPP32; Protein Yama; EC 3.4.22.56; Caspase-3; CASP-3; CPP-32; SCA-1; Yama; PARP Cleavage Protease; Procaspase3; EC 3.4.22

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:100
IHC1:50 - 1:100
IP1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12367 Mouse CASP3

GeneID: 25402 Rat CASP3

GeneID: 836 Human CASP3

Gene Symbol CASP3
Gene Full Name caspase 3, apoptosis-related cysteine peptidase
Background The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]
Function Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface. [UniProt]
Cellular Localization Cytoplasm. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30105 Apoptosis Marker Antibody Duo (Caspase3, PARP)
ARG30110 Mitochondria/Caspase dependant Apoptosis Antibody Panel (Caspase3, Caspase9, Cytochrome c, PARP) (WB)
Related products:
Caspase 3 antibodies; Caspase 3 Duos / Panels;
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
Calculated MW 32 kDa
PTM Acetylation, Phosphoprotein, S-nitrosylation, Zymogen. [UniProt]

Images (4) Click the Picture to Zoom In

  • ARG54938 anti-Caspase 3 antibody WB image

    Western blot: 20 µg of Mouse brain and Rat brain lysates stained with ARG54938 anti-Caspase 3 antibody at 1:250 dilution.

  • ARG54938 anti-Caspase 3 antibody ICC/IF image

    Immunofluorescence: NIH/3T3 cells stained with ARG54938 anti-Caspase 3 antibody at 1:100 dilution. Blue: DAPI for nuclear staining.

  • ARG54938 anti-Caspase 3 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human placenta stained with ARG54938 anti-Caspase 3 antibody at 1:100 dilution.

  • ARG54938 anti-Caspase 3 antibody WB image

    Western blot: 20 µg of 293T, and HeLa cell lysates stained with ARG54938 anti-Caspase 3 antibody at 1:500 dilution.

Customer's Feedback

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Review for anti-Caspase 3 antibody

Application:WB

Sample:Mouse brain and Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:250

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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Review for anti-Caspase 3 antibody

Application:WB

Sample:HeLa without / with Staurosporine

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:250

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

Review for anti-Caspase 3 antibody

Application:WB

Sample:1) Mouse spleen, 2) Rat spleen

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

Review for anti-Caspase 3 antibody

Application:WB

Sample:HeLa

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-Caspase 3 antibody

Application:WB

Sample:HaCaT cell

Sample Loading Amount:40μg

Primary Antibody Dilution Factor:1:1,000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Magnetic stirring with iron oxide nanospinners accretes neurotoxic Aβ42 oligomers into phagocytic clearable plaques for Alzheimer's disease treatment

ICC/IF, IHC-Fr / Mouse

Arjun Sabu et al.
Mater Today Bio.,  (2024)

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MiR-7-5p/KLF4 signaling inhibits stemness and radioresistance in colorectal cancer

WB / Human

Yuanyuan Shang et al.
Cell Death Discov.,  (2023)

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Human amniotic fluid mesenchymal stem cells attenuate pancreatic cancer cell proliferation and tumor growth in an orthotopic xenograft mouse model

WB / Human

Ying-Cheng Chen et al.
Stem Cell Res Ther,  (2022)

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Down-regulation of calreticulin promotes apoptosis in hepatic stellate cells.

WB / Human

Linyu Dai et al.
第三军医大学学报,  (2021)

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Multi-omics analysis of pathological changes in the amygdala of rats subjected to chronic restraint stress.

WB / Rat

Zhonghua Li et al.
Behav Brain Res.,  (2020)

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A LY-15, a novel cyclic pentapeptide that inhibits B16 cell proliferation and migration and induces cell apoptosis

WB / Mouse

Xuan Wang et al.
Oncol Lett.,  (2018)

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