ARG54938
anti-Caspase 3 antibody
anti-Caspase 3 antibody for ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
Publication5
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Caspase 3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Caspase 3 |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human Caspase-3 (NP_004337.2) |
| Conjugation | Un-conjugated |
| Alternate Names | CASP3; Caspase 3; Apopain; CPP32; CPP32B; Caspase 3, Apoptosis-Related Cysteine Peptidase; Caspase 3, Apoptosis-Related Cysteine Protease; SREBP Cleavage Activity 1; Cysteine Protease CPP32; Protein Yama; EC 3.4.22.56; Caspase-3; CASP-3; CPP-32; SCA-1; Yama; PARP Cleavage Protease; Procaspase3; EC 3.4.22 |
Application Instructions
| Application Suggestion |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CASP3 |
| Gene Full Name | caspase 3, apoptosis-related cysteine peptidase |
| Background | The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017] |
| Function | Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30105 Apoptosis Marker Antibody Duo (Caspase3, PARP) ARG30110 Mitochondria/Caspase dependant Apoptosis Antibody Panel (Caspase3, Caspase9, Cytochrome c, PARP) (WB) Related products: Caspase 3 antibodies; Caspase 3 Duos / Panels; |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody |
| Calculated MW | 32 kDa |
| PTM | Acetylation, Phosphoprotein, S-nitrosylation, Zymogen. [UniProt] |
Images (4) Click the Picture to Zoom In
-
ARG54938 anti-Caspase 3 antibody WB image
Western blot: 20 µg of Mouse brain and Rat brain lysates stained with ARG54938 anti-Caspase 3 antibody at 1:250 dilution.
-
ARG54938 anti-Caspase 3 antibody ICC/IF image
Immunofluorescence: NIH/3T3 cells stained with ARG54938 anti-Caspase 3 antibody at 1:100 dilution. Blue: DAPI for nuclear staining.
-
ARG54938 anti-Caspase 3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta stained with ARG54938 anti-Caspase 3 antibody at 1:100 dilution.
-
ARG54938 anti-Caspase 3 antibody WB image
Western blot: 20 µg of 293T, and HeLa cell lysates stained with ARG54938 anti-Caspase 3 antibody at 1:500 dilution.
Customer's Feedback
Good
Review for anti-Caspase 3 antibody
Application：WB
Sample：Mouse brain and Rat brain
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:250
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-Caspase 3 antibody
Application：WB
Sample：HeLa without / with Staurosporine
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:250
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
Review for anti-Caspase 3 antibody
Application：WB
Sample：1) Mouse spleen, 2) Rat spleen
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
Review for anti-Caspase 3 antibody
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-Caspase 3 antibody
Application：WB
Sample：HaCaT cell
Sample Loading Amount：40μg
Primary Antibody Dilution Factor：1:1,000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
MiR-7-5p/KLF4 signaling inhibits stemness and radioresistance in colorectal cancer
WB / Human
Human amniotic fluid mesenchymal stem cells attenuate pancreatic cancer cell proliferation and tumor growth in an orthotopic xenograft mouse model
WB / Human
Down-regulation of calreticulin promotes apoptosis in hepatic stellate cells.
WB / Human
Multi-omics analysis of pathological changes in the amygdala of rats subjected to chronic restraint stress.
WB / Rat
A LY-15, a novel cyclic pentapeptide that inhibits B16 cell proliferation and migration and induces cell apoptosis
WB / Mouse
