ARG10451
anti-Cardiac Troponin T antibody [1F11]
anti-Cardiac Troponin T antibody [1F11] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Bovine,Cat,Dog,Fish,Goat,Pig,Rabbit
Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [1F11] recognizes Cardiac Troponin T |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov, Cat, Dog, Fsh, Goat, Pig, Rb |
| Tested Application | ELISA, FACS, ICC/IF, IHC-P, WB |
| Specificity | No cross-reaction with skeletal TnT. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1F11 |
| Isotype | IgG2b |
| Target Name | Cardiac Troponin T |
| Antigen Species | Human |
| Immunogen | free human cTnT |
| Epitope | within a.a. 145-164 |
| Conjugation | Un-conjugated |
| Alternate Names | RCM3; CMPD2; LVNC6; TnTC; TnTc; CMH2; cTnT; Cardiac muscle troponin T; CMD1D; Troponin T, cardiac muscle |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A affinity purified. |
| Buffer | PBS (pH 7.4) and 0.1% Sodium azide |
| Preservative | 0.1% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TNNT2 |
| Gene Full Name | troponin T type 2 (cardiac) |
| Background | The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008] |
| Function | Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody |
| Calculated MW | 36 kDa |
| PTM | Phosphorylation at Thr-213 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity. |
Images (3) Click the Picture to Zoom In
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ARG10451 anti-Cardiac Troponin T antibody [1F11] WB image
Western blot: 20 μg of Rat heart lysate stained with ARG10451 anti-Cardiac Troponin T antibody [1F11] at 1:416 dilution.
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ARG10451 anti-Cardiac Troponin T antibody [1F11] IHC-P image
Immunohistochemistry: Paraffin-embedded ischemic pig heart tissue stained with ARG10451 anti-Cardiac Troponin T antibody [1F11].
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ARG10451 anti-Cardiac Troponin T antibody [1F11] WB image
Western blot: Bovine muscle, heart, liver, kidney and lung lysates stained with ARG10451 anti-Cardiac Troponin T antibody [1F11].
Bovine heart serves as a positive control; Other tissue samples serve as negative controls.
Customer's Feedback
Excellent
Review for anti-Cardiac Troponin T antibody [1F11]
Application：WB
Sample：Rat heart
Sample Loading Amount：20 μg
Primary Antibody Dilution Factor：1:416
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-Cardiac Troponin T antibody [1F11]
Application：WB
Sample：Mouse heart
Sample Loading Amount：20 μg
Primary Antibody Dilution Factor：1:416
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Clone References
Differential activation of natriuretic peptide receptors modulates cardiomyocyte proliferation during development.
Zebularine regulates early stages of mESC differentiation: effect on cardiac commitment.
WB / Mouse
High density sphere culture of adult cardiac cells increases the levels of cardiac and progenitor markers and shows signs of vasculogenesis.
IHC / Human
Transplantation of parthenogenetic embryonic stem cells ameliorates cardiac dysfunction and remodelling after myocardial infarction.
IHC-P / Mouse
Conversion of mouse fibroblasts into cardiomyocytes using a direct reprogramming strategy.
FACS / Mouse
Conditional transgenic expression of fibroblast growth factor 9 in the adult mouse heart reduces heart failure mortality after myocardial infarction.
ICC/IF, IHC-Fr / Mouse
Assaying cardiac biomarkers for toxicity testing using biosensing and cardiomyocytes derived from human embryonic stem cells.
IA / Human
