ARG40277
anti-Calpain 1 antibody
anti-Calpain 1 antibody for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Calpain 1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Hrs |
| Tested Application | ICC/IF, IHC-Fr, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Calpain 1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 312-326 of Human Calpain 1. (EWNNVDPYERDQLRV) |
| Conjugation | Un-conjugated |
| Alternate Names | EC 3.4.22.52; CANP1; Calpain-1 large subunit; Calpain-1 catalytic subunit; Calpain mu-type; muCANP; Cell proliferation-inducing gene 30 protein; Micromolar-calpain; CANP 1; CANP; muCL; CANPL1; Calcium-activated neutral proteinase 1 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 80 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Thimerosal and 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CAPN1 |
| Gene Full Name | calpain 1, (mu/I) large subunit |
| Background | The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010] |
| Function | Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. [UniProt] |
| Cellular Localization | Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+) binding. In granular keratinocytes and in lower corneocytes, colocalizes with FLG and FLG2 (PubMed:21531719). [UniProt] |
| Calculated MW | 82 kDa |
| PTM | Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms. [UniProt] |
Images (8) Click the Picture to Zoom In
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ARG40277 anti-Calpain 1 antibody ICC/IF image
Immunocytochemistry: HeLa cells stained with ARG40277 anti-Calpain 1 antibody.
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ARG40277 anti-Calpain 1 antibody ICC/IF image
Immunofluorescence: A549 cells were blocked with 10% goat serum and then stained with ARG40277 anti-Calpain 1 antibody (green) at 5 µg/ml dilution, overnight at 4°C.
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ARG40277 anti-Calpain 1 antibody IHC-Fr image
Immunohistochemistry: Frozen section of Rat cardiac muscle tissue. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40277 anti-Calpain 1 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG40277 anti-Calpain 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat lung stained with ARG40277 anti-Calpain 1 antibody.
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ARG40277 anti-Calpain 1 antibody WB image
Western blot: HT1080 and COLO320 cell lysates stained with ARG40277 anti-Calpain 1 antibody.
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ARG40277 anti-Calpain 1 antibody WB image
Western blot: 50 µg of sample under reducing conditions. Mouse spleen, Mouse lung, Mouse kidney, Mouse brain, Mouse thymus, Mouse small intestine and Mouse ovary lysates stained with ARG40277 anti-Calpain 1 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG40277 anti-Calpain 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer stained with ARG40277 anti-Calpain 1 antibody.
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ARG40277 anti-Calpain 1 antibody WB image
Western blot: 50 µg of sample under reducing conditions. Rat brain, Rat heart, Rat testis, Rat thymus, MDA-MB-231, HeLa, SMMC-7721, HT1080 and Colo320 whole cell lysates stained with ARG40277 anti-Calpain 1 antibody at 0.5 µg/ml dilution, overnight at 4°C.
