ARG10702
anti-Calbindin antibody [5A9]
anti-Calbindin antibody [5A9] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow,Horse,Pig
Overview
| Product Description | Mouse Monoclonal antibody [5A9] recognizes Calbindin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Cow, Hrs, Pig |
| Predict Reactivity | Chk |
| Tested Application | ICC/IF, IHC-Fr, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 5A9 |
| Isotype | IgG2a |
| Target Name | Calbindin |
| Antigen Species | Human |
| Immunogen | Full length recombinant Human protein. |
| Conjugation | Un-conjugated |
| Alternate Names | Vitamin D-dependent calcium-binding protein, avian-type; Calbindin; CALB; Calbindin D28; D-28K |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification. |
| Buffer | PBS and 50% Glycerol. |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CALB1 |
| Gene Full Name | calbindin 1, 28kDa |
| Background | The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. [provided by RefSeq, Jan 2015] |
| Function | Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. [UniProt] |
| Highlight | Related products: Calbindin antibodies; Anti-Mouse IgG secondary antibodies; Related news: Viral-like capsids, new trans-synaptic mRNA transport mechanism |
| Calculated MW | 30 kDa |
Images (5) Click the Picture to Zoom In
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ARG10702 anti-Calbindin antibody [5A9] IHC-Fr image
Immunohistochemistry: Frozen section of Rat brain cerebellum stained with ARG10702 anti-Calbindin antibody [5A9] (red) at 1:2000 dilution and costained with ARG52312 anti-GFAP antibody (green) at 1:5000 dilution. DAPI (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with the above antibodies.)
The Clone 5A9 calbindin antibody prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The GFAP antibody stains the processes of Bergmann glia in the molecular layer and astroglia in the granular and white matter layers of cerebellum.
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ARG10702 anti-Calbindin antibody [5A9] IHC-Fr image
Immunohistochemistry: Frozen sections of adult Mouse cortex were stained with ARG10702 anti-Calbindin antibody [5A9] (red), and co-stained with our rabbit polyclonal anti-Fox3 / NeuN antibody (green). Calbindin is expressed in a subset of interneurons in the cortex. Fox3 / NeuN expresses in most neurons; as a result, cells positive for calbindin appear to be yellow. The Inset is a high magnification image of the boxed area. Blue is DAPI nucleus staining that labels DNA.
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ARG10702 anti-Calbindin antibody [5A9] WB image
Western blot: Cow cerebellum lysate were stained with ARG10702 anti-Calbindin antibody [5A9].
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ARG10702 anti-Calbindin antibody [5A9] WB image
Western blot: Rat cerebellum, Pig hippocampus and Cow cerebellum lysates stained with ARG10702 anti-Calbindin antibody [5A9] (green) at 1:5000 dilution.
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ARG10702 anti-Calbindin antibody [5A9] IHC-Fr image
Immunohistochemistry: Frozen sections of adult Mouse cerebellum were stained with ARG10702 anti-Calbindin antibody [5A9] (red), and co-stained with our rabbit polyclonal anti-Fox3 / NeuN antibody (green). Calbindin is prominently expressed in the dendrites of Purkinje cells in the cerebellum molecular layer. Fox3 / NeuN expresses in most neurons; as a result, cells positive for calbindin appear to be yellow. The Inset is a high magnification image of the boxed area. Blue is DAPI nucleus staining that labels DNA.
