ARG56181
anti-Cadherin 16 antibody [CDH16/1071]
anti-Cadherin 16 antibody [CDH16/1071] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [CDH16/1071] recognizes Cadherin 16 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | CDH16/1071 |
| Isotype | IgG1, kappa |
| Target Name | Cadherin 16 |
| Antigen Species | Human |
| Immunogen | Recombinant human Cadherin-16 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | Kidney-specific cadherin; Ksp-cadherin; Cadherin-16 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Human kidney | ||||||||||
| Observed Size | ~ 105 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CDH16 |
| Gene Full Name | cadherin 16, KSP-cadherin |
| Background | This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. Mapped to a previously identified cluster of cadherin genes on chromosome 16q22.1, the gene localizes with superfamily members CDH1, CDH3, CDH5, CDH8 and CDH11. The protein consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011] |
| Function | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. [UniProt] |
| Cellular Localization | Cell surface with some cytoplasmic |
| Calculated MW | 90 kDa |
Images (7) Click the Picture to Zoom In
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ARG56181 anti-Cadherin 16 antibody [CDH16/1071] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human renal cell carcinoma stained with ARG56181 anti-Cadherin 16 antibody [CDH16/1071].
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ARG56181 anti-Cadherin 16 antibody [CDH16/1071] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Mouse kidney tissue. Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT for 20 min. The tissue section was stained with ARG56181 anti-Cadherin 16 antibody [CDH16/1071].
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ARG56181 anti-Cadherin 16 antibody [CDH16/1071] WB image
Western blot: Human kidney lysate stained with ARG56181 anti-Cadherin 16 antibody [CDH16/1071].
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ARG56181 anti-Cadherin 16 antibody [CDH16/1071] FACS image
Flow Cytometry: HEK293 cells stained with ARG56181 anti-Cadherin 16 antibody [CDH16/1071] (blue); Isotype control (red).
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ARG56181 anti-Cadherin 16 antibody [CDH16/1071] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Rat kidney stained with ARG56181 anti-Cadherin 16 antibody [CDH16/1071].
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ARG56181 anti-Cadherin 16 antibody [CDH16/1071] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human kidney tissue. Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT for 20 min. The tissue section was stained with ARG56181 anti-Cadherin 16 antibody [CDH16/1071].
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ARG56181 anti-Cadherin 16 antibody [CDH16/1071] IHC image
Immunohistochemistry: Human kidney tissue stained with ARG56181 anti-Cadherin 16 antibody [CDH16/1071] (purple).
