ARG20520
anti-CaMKII phospho (Thr286) antibody [22B1]
anti-CaMKII phospho (Thr286) antibody [22B1] for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody
Publication1
Overview
| Product Description | Mouse Monoclonal antibody [22B1] recognizes CaMKII phospho (Thr286) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ELISA, ICC/IF, IHC-P, IP, WB |
| Specificity | Detects phosphorylated CaMKII from rat tissues. Monoclonal antibody 22B1 (anti-phosphoCaMKII is specific for α and β subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in β). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 22B1 |
| Isotype | IgG |
| Target Name | CaMKII |
| Antigen Species | Rat |
| Immunogen | Phosphospecific peptide around Thr286 of Rat CaMKII (NP_037052.1). |
| Conjugation | Un-conjugated |
| Alternate Names | CAMKA; CaMK-II subunit alpha; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; EC 2.7.11.17 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||
| Positive Control | Rat brain tissue extract. | ||||||||||||
| Observed Size | ~50 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G affinity purified |
| Buffer | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Camk2a |
| Gene Full Name | calcium/calmodulin-dependent protein kinase II alpha |
| Background | The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008] |
| Function | CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. [UniProt] |
| Highlight | Related products: anti-CaMKII phospho (Thr286) antibody [22B1]; |
| Research Area | Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 54 kDa |
Images (5) Click the Picture to Zoom In
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ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1] IHC-P image
Immunohistochemistry: Bouin's fixed paraffin-embedded backskin sections of transgenic mice- muscle, and hair follicle stained with ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1].
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ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1] WB image
Western blot: Rat brain lysate stained with ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1].
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ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1] ICC/IF image
Immunofluorescence: Rat hippocampal neurons stained with ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1].
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ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1] IHC image
Immunohistochemistry: Formalin-fixed Human colon carcinoma stained with ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1] at 1:5000 for 12 hours at 4°C. Secondary antibody: Biotin Goat anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 min at RT. Magnification: 40x.
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ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1] WB image
Western blot: 1) Ventricles lysate from 14-Day-Old normal Mouse, 2) Ventricles lysate from 14-Day-Old CaM overexpressing transgenic Mouse line 2295, 3) Ventricles lysate from 14-Day-Old CaM-8 (mutant CaM) expressing transgenic Mouse line 4466, stained with ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1]. (PMID: 10935538).
Specific References
Consuming oxidative frying oil impairs cardiac energy production and calcium recycling, causing cardiac hypertrophy, fibrosis and diastolic dysfunction in male Sprague Dawley rats
WB / Rat
