ARG64934
anti-CaMKII alpha antibody
anti-CaMKII alpha antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes CaMKII alpha |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, WB |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_057065.2; NP_741960.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CaMKII alpha |
| Antigen Species | Human |
| Immunogen | C-PRTAQSEETRVWHR |
| Conjugation | Un-conjugated |
| Alternate Names | CAMKA; CaMK-II subunit alpha; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; EC 2.7.11.17 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008] |
| Highlight | Related Antibody Duos and Panels: ARG30157 Phospho CaMKII Antibody Duo Related products: CaMKII alpha antibodies; CaMKII alpha Duos / Panels; Anti-Goat IgG secondary antibodies; |
| Research Area | Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 54 kDa |
Images (5) Click the Picture to Zoom In
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ARG64934 anti-CaMKII alpha antibody WB image
Western blot: Mouse Brain lysate (35 µg protein in RIPA buffer) stained with ARG64934 anti-CaMKII alpha antibody at 0.1 µg/ml dilution.
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ARG64934 anti-CaMKII alpha antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG64934 anti-CaMKII alpha antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG64934 anti-CaMKII alpha antibody WB image
Western blot: 35 µg of Human cerebellum (A), Mouse brain (B) and Rat brain (C) lysates (in RIPA buffer) stained with ARG64934 anti-CaMKII alpha antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
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ARG64934 anti-CaMKII alpha antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed Kelly cells permeabilized with 0.5% Triton. Cells were stained with ARG64934 anti-CaMKII alpha antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
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ARG64934 anti-CaMKII alpha antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U251 cells permeabilized with 0.15% Triton. Cells were stained with ARG64934 anti-CaMKII alpha antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
