ARG22260
anti-CaMKII alpha antibody [6G9]
anti-CaMKII alpha antibody [6G9] for ELISA,ICC/IF,Immunohistochemistry,Immunoprecipitation,Radioimmunoassay,Western blot and Human,Mouse,Rat,Bovine
Publication1
Overview
| Product Description | Mouse Monoclonal antibody [6G9] recognizes CaMKII alpha |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov |
| Tested Application | ELISA, ICC/IF, IHC, IP, RIA, WB |
| Specificity | Detects ~50-60kDa. Recognizes both phosphorylated and non-phosphorylated forms. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 6G9 |
| Isotype | IgG1 |
| Target Name | CaMKII alpha |
| Antigen Species | Rat |
| Immunogen | Partially purified Rat CaMKII |
| Conjugation | Un-conjugated |
| Alternate Names | CAMKA; CaMK-II subunit alpha; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; EC 2.7.11.17 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Camk2a |
| Gene Full Name | calcium/calmodulin-dependent protein kinase II alpha |
| Background | The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008] |
| Function | CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity (By similarity). [UniProt] |
| Cellular Localization | Cytoplasm, Cell Junction, Mitochondrion, Nucleus, Presynaptic Cell Membrane, Synapse |
| Calculated MW | 54 kDa |
Images (4) Click the Picture to Zoom In
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ARG22260 anti-CaMKII alpha antibody [6G9] ICC/IF image
Immunocytochemistry: Mouse dissociated hippocampal neurons stained with ARG22260 anti-CaMKII alpha antibody [6G9] at 1:1000 dilution.
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ARG22260 anti-CaMKII alpha antibody [6G9] WB image
Western blot: Rat brain lysate stained with ARG22260 anti-CaMKII alpha antibody [6G9].
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ARG22260 anti-CaMKII alpha antibody [6G9] IHC image
Immunohistochemistry: Formalin fixed colon carcinoma stained with ARG22260 anti-CaMKII alpha antibody [6G9] at 1:10000 dilution (12 hours). Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200uL for 2 min at RT. Magnification: 40x.
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ARG22260 anti-CaMKII alpha antibody [6G9] WB image
Western blot: Rat brain membrane lysate stained with ARG22260 anti-CaMKII alpha antibody [6G9] at 1:1000 dilution.
Specific References
Consuming oxidative frying oil impairs cardiac energy production and calcium recycling, causing cardiac hypertrophy, fibrosis and diastolic dysfunction in male Sprague Dawley rats
WB / Rat
