ARG66326
anti-COX4 antibody [SQab1874]
anti-COX4 antibody [SQab1874] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,African green monkey,Bovine,Chicken,Dog,Pig
Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody; Loading Control antibody for Cytoplasmic Fractions; Cytochrome-C fractionation Study antibody; Mitochondrial Marker antibody
Publication1
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab1874] recognizes COX4 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, AGMK, Bov, Chk, Dog, Pig |
| Tested Application | FACS, ICC/IF, IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab1874 |
| Isotype | IgG |
| Target Name | COX4 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the N-terminus of Human COX IV. |
| Conjugation | Un-conjugated |
| Alternate Names | Cytochrome c oxidase polypeptide IV; COX4; COX4-1; COXIV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | COX4I1 |
| Gene Full Name | cytochrome c oxidase subunit IV isoform 1 |
| Background | Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016] |
| Function | COX4 protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. [UniProt] |
| Research Area | Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody; Loading Control antibody for Cytoplasmic Fractions; Cytochrome-C fractionation Study antibody; Mitochondrial Marker antibody |
| Calculated MW | 20 kDa |
Images (7) Click the Picture to Zoom In
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ARG66326 anti-COX4 antibody [SQab1874] ICC/IF image
Immunofluorescence: HeLa cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66326 anti-COX4 antibody [SQab1874] (green) at 1:50 and 4°C. DAPI (blue) was used as the nuclear counter stain.
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ARG66326 anti-COX4 antibody [SQab1874] FACS image
Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66326 anti-COX4 antibody [SQab1874] (red) at 1:1,000 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
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ARG66326 anti-COX4 antibody [SQab1874] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded liver tissue stained with ARG66326 anti-COX4 antibody [SQab1874] at 1:200 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
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ARG66326 anti-COX4 antibody [SQab1874] WB image
Western blot: 10 µg of A431, 293, HeLa and Raw264.7 cell lysates stained with ARG66326 anti-COX4 antibody [SQab1874] at 1:5000 dilution.
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ARG66326 anti-COX4 antibody [SQab1874] IP image
Immunoprecipitation: 0.4 mg of HeLa whole cell lysate immunoprecipitated (1:50) and stained with ARG66326 anti-COX4 antibody [SQab1874]. 1) ARG66326 IP in HeLa whole cell lysate, 2) PBS instead of ARG66326 in HeLa whole cell lysate, and 3) HeLa whole cell lysate, 10 µg (input).
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ARG66326 anti-COX4 antibody [SQab1874] WB image
Western blot: 10 µg of MDBK, MDCK, Cos-7, Chicken heart and Pig heart lysates stained with ARG66326 anti-COX4 antibody [SQab1874] at 1:5000 dilution.
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ARG66326 anti-COX4 antibody [SQab1874] WB image
Western blot: 2 µg of Mouse heart, Mouse kidney, Mouse liver, Rat heart, Rat kidney and Rat liver lysates stained with ARG66326 anti-COX4 antibody [SQab1874] at 1:5000 dilution.
Specific References
TRK-fused gene (TFG) regulates ULK1 stability via TRAF3-mediated ubiquitination and protects macrophages from LPS-induced pyroptosis
WB / Human
