ARG53919
anti-CLTC / Clathrin heavy chain antibody [BF-06]
anti-CLTC / Clathrin heavy chain antibody [BF-06] for ELISA,Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat,Bovine,Pig
Controls and Markers antibody; Signaling Transduction antibody; Endosome Marker antibody
Publication1
Overview
| Product Description | Mouse Monoclonal antibody [BF-06] recognizes CLTC / Clathrin heavy chain |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov, Pig |
| Tested Application | ELISA, FACS, ICC/IF, IP, WB |
| Specificity | The clone BF-06 recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa protein involved in receptor-mediated endocytosis. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | BF-06 |
| Isotype | IgM |
| Target Name | CLTC / Clathrin heavy chain |
| Conjugation | Un-conjugated |
| Alternate Names | CHC17; CHC; Clathrin heavy chain 1; Hc; CLH-17; CLTCL2; Clathrin heavy chain on chromosome 17 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||
| Positive Control | FACS: Human blood leukocytes |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by precipitation methods and ion exchange chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | TBS (pH 8.0) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CLTC |
| Gene Full Name | clathrin, heavy chain (Hc) |
| Background | Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results in membrane invagination. The basic oligomers are composed of three clathrin heavy chain (180 kDa) and three light chain (30 kDa) subunits and the process of polymerization is dynamically regulated by the light chains. Interaction of clathrin with the plasma membrane is mediated by adaptor proteins (AP1-4) specific for different cellular compartments. Another proteins, such as endophilin, epsin and amphiphysin are involved in membrane invagination and clathrin rearrangements. Finally, dynamin functions at the fission stage of clathrin-mediated endocytosis. |
| Function | Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30310 Endosome, Lysosome, Peroxisome Marker Antibody Panel (Catalase, Caveolin1, Clathrin heavy chain, LAMP1) Related products: Clathrin antibodies; Clathrin Duos / Panels; Anti-Mouse IgM secondary antibodies; Related poster download: Organelle Markers & Loading Control |
| Research Area | Controls and Markers antibody; Signaling Transduction antibody; Endosome Marker antibody |
| Calculated MW | 192 kDa |
Images (2) Click the Picture to Zoom In
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ARG53919 anti-CLTC / Clathrin heavy chain antibody [BF-06] ICC/IF image
Immunofluorescence: Human primary fibroblasts stained with ARG53919 anti-CLTC / Clathrin heavy chain antibody [BF-06] (green).
Actin cytoskeleton was stained with phalloidin (red) and cell nuclei stained with DAPI (blue). -
ARG53919 anti-CLTC / Clathrin heavy chain antibody [BF-06] ICC/IF image
Immunofluorescence: HeLa cells stained with ARG53919 anti-CLTC / Clathrin heavy chain antibody [BF-06] (green).
Actin cytoskeleton was stained with phalloidin (red) and cell nuclei stained with DAPI (blue).
Specific References
Synaptic endocytosis in adult adipose stromal cell-derived neurons
ICC/IF / Human
