Overview

Product Description	Rabbit Polyclonal antibody recognizes CHD3
Tested Reactivity	Hu, Ms, Rat
Tested Application	FACS, ICC/IF, IHC-P, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	CHD3
Antigen Species	Human
Immunogen	Synthetic peptide of Human CHD3.
Conjugation	Un-conjugated
Alternate Names	Zinc finger helicase; Mi2-ALPHA; Chromodomain-helicase-DNA-binding protein 3; hZFH; Mi2-alpha; Mi-2a; Mi-2 autoantigen 240 kDa protein; ZFH; EC 3.6.4.12; ATP-dependent helicase CHD3; CHD-3

Application Instructions

Application Suggestion	Tested Application Dilution FACS 1:50 ICC/IF 1:50 - 1:200 IHC-P 1:50 - 1:200 WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	K562
Observed Size	~ 250 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 1107 Human CHD3 Swiss-port # Q12873 Human Chromodomain-helicase-DNA-binding protein 3
Gene Symbol	CHD3
Gene Full Name	chromodomain helicase DNA binding protein 3
Background	This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Function	Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity. [UniProt]
Cellular Localization	Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Associates with centrosomes in interphase and mitosis. [UniProt]
Calculated MW	227 kDa

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