ARG66244
anti-CDX2 antibody [SQab1733]
anti-CDX2 antibody [SQab1733] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab1733] recognizes CDX2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab1733 |
| Isotype | IgG |
| Target Name | CDX2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the N-terminus of Human CDX2. |
| Conjugation | Un-conjugated |
| Alternate Names | CDX2/AS; CDX3; Homeobox protein CDX-2; CDX-3; Caudal-type homeobox protein 2 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | SW480; Human colon tissue | ||||||
| Observed Size | ~ 40 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CDX2 |
| Gene Full Name | caudal type homeobox 2 |
| Background | This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded protein is a major regulator of intestine-specific genes involved in cell growth an differentiation. This protein also plays a role in early embryonic development of the intestinal tract. Aberrant expression of this gene is associated with intestinal inflammation and tumorigenesis. [provided by RefSeq, Jan 2012] |
| Function | Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. [UniProt] |
| Highlight | Related products: CDX2 antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones) |
| Calculated MW | 34 kDa |
| PTM | Phosphorylation of Ser-60 mediates the transactivation capacity. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG66244 anti-CDX2 antibody [SQab1733] WB image (Customer's Feedback)
Western blot: SW480 cell lysate stained with ARG66244 anti-CDX2 antibody [SQab1733] at 1:1000 dilution.
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ARG66244 anti-CDX2 antibody [SQab1733] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human colon tissue stained with ARG66244 anti-CDX2 antibody [SQab1733] at 1:200. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
Customer's Feedback
Excellent
Review for anti-CDX2 antibody [SQab1733]
Application：WB
Sample：SW480
Sample Loading Amount：20 μg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：Overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：HRP
