ARG62960
anti-CDK1 / CDC2 antibody [POH-1]
anti-CDK1 / CDC2 antibody [POH-1] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Bovine,Primates
Cell Biology and Cellular Response antibody; Gene Regulation antibody; Neuroscience antibody
Publication1
Overview
| Product Description | Mouse Monoclonal antibody [POH-1] recognizes CDK1 / CDC2 |
|---|---|
| Tested Reactivity | Hu, Bov, NHuPrm |
| Species Does Not React With | Ms, Rat, Dm, Xenopus laevis |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Specificity | The clone POH-1 specifically detects Cdk1 (p34Cdc2). Staining of Cdk1 reflects the proliferating potential of respective tissue. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | POH-1 |
| Isotype | IgG2a |
| Target Name | CDK1 / CDC2 |
| Antigen Species | Human |
| Immunogen | Bacterially expressed full-length human Cdk1 protein |
| Conjugation | Un-conjugated |
| Alternate Names | CDK1; Cyclin Dependent Kinase 1; CDC28A; CDC2; Cell Division Cycle 2, G1 To S And G2 To M; Cell Division Control Protein 2 Homolog; Cell Division Protein Kinase 1; Cyclin-Dependent Kinase 1; P34 Protein Kinase; P34CDC2; Cell Cycle Controller CDC2; EC 2.7.11.22; EC 2.7.11.23; CDKN1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Positive Control | IHC-P: Thymus tissue. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CDK1 |
| Gene Full Name | cyclin-dependent kinase 1 |
| Background | The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2023] |
| Function | Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition via association with multiple interphase cyclins. [UniProt] |
| Cellular Localization | Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Gene Regulation antibody; Neuroscience antibody |
| Calculated MW | 34 kDa |
| PTM | Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG62960 anti-CDK1 / CDC2 antibody [POH-1] WB image
Western blot: 20 µg of Jurkat and HeLa cell lysates stained with ARG62960 anti-CDK1 / CDC2 antibody [POH-1] at 1:1000 dilution.
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ARG62960 anti-CDK1 / CDC2 antibody [POH-1] IHC-P image
Immunohistochemistry: Human thymus (paraffin sections) stained with ARG62960 anti-CDK1 / CDC2 antibody [POH-1].
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Review for anti-CDK1 / CDC2 antibody [POH-1]
Application：WB
Sample：Jurkat and HeLa
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
A novel mechanism driving poor-prognostic gastric cancer: overexpression of the transcription factor Krüppel-like factor 16 promotes growth and metastasis of gastric cancer through regulating the Notch pathway.
WB / Human
