ARG66203
anti-CD99 antibody [SQab1725]
anti-CD99 antibody [SQab1725] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab1725] recognizes CD99 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab1725 |
| Isotype | IgG |
| Target Name | CD99 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the C‐terminus of CD99. |
| Conjugation | Un-conjugated |
| Alternate Names | 12E7; CD99 antigen; MIC2X; MIC2Y; CD antigen CD99; MSK5X; Protein MIC2; MIC2; T-cell surface glycoprotein E2; HBA71; E2 antigen |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed using Citrate buffer (pH 6.0) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD99 |
| Gene Full Name | CD99 molecule |
| Background | CD99 is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus. [provided by RefSeq, Mar 2016] |
| Function | CD99 involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes. [UniProt] |
| Highlight | Related products: CD99 antibodies; Related news: Cancer Pathology Markers (SQ clones) |
| Calculated MW | 19 kDa |
| PTM | Extensively O-glycosylated. |
Images (3) Click the Picture to Zoom In
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ARG66203 anti-CD99 antibody [SQab1725] WB image
Western blot: 30 µg of HeLa and Molt4 cell lysates stained with ARG66203 anti-CD99 antibody [SQab1725] at 1:500 dilution.
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ARG66203 anti-CD99 antibody [SQab1725] IHC-P image
Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human pancreas tissue stained with ARG66203 anti-CD99 antibody [SQab1725] at 1:500 dilution.
Antigen retrieval: Heat mediated was performed using Citrate buffer pH 6.0
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ARG66203 anti-CD99 antibody [SQab1725] IHC-P image
Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human tonsil tissue stained with ARG66203 anti-CD99 antibody [SQab1725] at 1:500 dilution.
Antigen retrieval: Heat mediated was performed using Citrate buffer pH 6.0
