ARG62948
anti-CD9 antibody [EM-04]
anti-CD9 antibody [EM-04] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Mouse
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody
Overview
| Product Description | Rat Monoclonal antibody [EM-04] recognizes CD9 |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | FACS, ICC/IF, IP, WB |
| Specificity | The clone EM-04 recognizes CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes. |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | EM-04 |
| Isotype | IgG1 |
| Target Name | CD9 |
| Antigen Species | Mouse |
| Immunogen | Permeabilized murine bone marrow-derived mast cells (BMMC). |
| Conjugation | Un-conjugated |
| Alternate Names | Leukocyte antigen MIC3; BTCC-1; TSPAN-29; MIC3; Tetraspanin-29; p24; Cell growth-inhibiting gene 2 protein; CD9 antigen; MRP-1; DRAP-27; 5H9 antigen; CD antigen CD9; TSPAN29; Motility-related protein; Tspan-29 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Under non-reducing condition. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by protein A |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Cd9 |
| Gene Full Name | CD9 antigen |
| Background | CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors. |
| Function | Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion (By similarity). Acts as a receptor for PSG17. [UniProt] |
| Highlight | Related products: CD9 antibodies; CD9 ELISA Kits; Anti-Rat IgG secondary antibodies; Related news: New antibodies for exosome isolation |
| Research Area | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody |
| Calculated MW | 25 kDa |
| PTM | Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro). The protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. |
Images (2) Click the Picture to Zoom In
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ARG62948 anti-CD9 antibody [EM-04] WB image
Western blot: Bone marrow-derived mast cell (BMMC) lysates stained with ARG62948 anti-CD9 antibody [EM-04].
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ARG62948 anti-CD9 antibody [EM-04] FACS image
Flow Cytometry: Mouse peritoneal fluid cells stained with ARG62948 anti-CD9 antibody [EM-04], followed by incubation with APC labelled Donkey anti-Rat secondary antibody.
