ARG56144
anti-CD83 antibody [HB15e]
anti-CD83 antibody [HB15e] for CyTOF®-candidate,Flow cytometry,IHC-Frozen sections and Human,Primates
Overview
| Product Description | Mouse Monoclonal antibody [HB15e] recognizes CD83 |
|---|---|
| Tested Reactivity | Hu, NHuPrm |
| Tested Application | CyTOF®-candidate, FACS, IHC-Fr |
| Specificity | This antibody recognizes CD83, a 40-45 kDa type I glycoprotein expressed on mature dendritic cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | HB15e |
| Isotype | IgG1 |
| Target Name | CD83 |
| Antigen Species | Human |
| Immunogen | Human CD83-transfected Cos cells. |
| Conjugation | Un-conjugated |
| Alternate Names | hCD83; CD83 antigen; CD antigen CD83; HB15; BL11; Cell surface protein HB15; B-cell activation protein |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-Fr: Acetone fixation * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | FACS: Peripheral blood-derived dendritic cells. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD83 |
| Gene Full Name | CD83 molecule |
| Background | The protein encoded by this gene is a single-pass type I membrane protein and member of the immunoglobulin superfamily of receptors. The encoded protein may be involved in the regulation of antigen presentation. A soluble form of this protein can bind to dendritic cells and inhibit their maturation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| Function | May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation. [UniProt] |
| Highlight | Related products: CD83 antibodies; Anti-Mouse IgG secondary antibodies; Related news: CyTOF-candidate Antibodies Detecting exosomal HMGB1 for ICD research |
| Calculated MW | 23 kDa |
Images (1) Click the Picture to Zoom In
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ARG56144 anti-CD83 antibody [HB15e] FACS image
Flow Cytometry: Separation of IFN alpha-stimulated human monocytes (red) from unstimulated peripheral blood mononuclear cells (black). Cells were stained with ARG56144 anti-CD83 antibody [HB15e] at 2 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
