ARG66253

anti-CD68 antibody [SQab1742]

Datasheet

Publication2

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100 μl

389.00

50 μl

272.00

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Datasheet
Publication (2)

Overview

Product Description	Recombinant Rabbit Monoclonal antibody [SQab1742] recognizes CD68
Tested Reactivity	Hu
Tested Application	FACS, IHC-Fr, IHC-P
Host	Rabbit
Clonality	Monoclonal
Clone	SQab1742
Isotype	IgG
Target Name	CD68
Antigen Species	Human
Immunogen	Synthetic peptide around aa. 100-200 of Human CD68.
Conjugation	Un-conjugated
Alternate Names	Macrosialin; CD antigen CD68; LAMP4; Gp110; GP110; SCARD1

Application Instructions

Application Suggestion

Tested Application	Dilution
FACS	1:25 - 1:100
IHC-Fr	Assay-dependent
IHC-P	1:100 - 1:200

Application Note

IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Purification with Protein A.
Buffer	PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative	0.01% Sodium azide
Stabilizer	40% Glycerol and 0.05% BSA
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 968 Human CD68 Swiss-port # P34810 Human Macrosialin
Gene Symbol	CD68
Gene Full Name	CD68 molecule
Background	CD68 is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
Function	CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. [UniProt]
Highlight	Related Antibody Duos and Panels: ARG30325 Inflammatory Cell Antibody Panel Related products: CD68 antibodies; CD68 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones) New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) Exploring Antiviral Immune Response Anti-SerpinB9 therapy, a new strategy for cancer therapy RIP1 activation and pathogenesis of NASH
Research Area	Immune System antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Active macroglial Marker antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody; Inflammatory Cell Marker antibody
Calculated MW	37 kDa
PTM	N- and O-glycosylated. [UniProt]

Images (2) Click the Picture to Zoom In

ARG66253 anti-CD68 antibody [SQab1742] FACS image

Flow Cytometry: THP-1 cells were fixed with 4% paraformaldehyde for 10 min. The cells were then stained with ARG66253 anti-CD68 antibody [SQab1742] (red) at 1:100 dilution in 1x PBS/1% BSA for 30 min at room temperature, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (Black) was used as a control.
ARG66253 anti-CD68 antibody [SQab1742] IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Human liver tissue stained with ARG66253 anti-CD68 antibody [SQab1742] at 1:200. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).