ARG23346
anti-CD63 antibody [TS63] (azide free)
anti-CD63 antibody [TS63] (azide free) for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Publication1
Overview
| Product Description | Azide free Mouse Monoclonal antibody [TS63] recognizes CD63 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P, IP, WB |
| Specificity | This antibody recognizes the LIMP antigen, a 30-60 kDa (smear) protein. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | TS63 |
| Isotype | IgG1 |
| Target Name | CD63 |
| Antigen Species | Human |
| Immunogen | Jurkat and HEL cell lines |
| Conjugation | Un-conjugated |
| Alternate Names | Tspan-30; CD63 antigen; Tetraspanin-30; CD antigen CD63; Lysosomal-associated membrane protein 3; OMA81H; Ocular melanoma-associated antigen; Granulophysin; TSPAN30; Melanoma-associated antigen ME491; MLA1; LAMP-3; ME491 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | Sterile-filtered through 0.22 µm. |
| Buffer | PBS |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD63 |
| Gene Full Name | CD63 molecule |
| Background | The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012] |
| Function | Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli. [UniProt] |
| Highlight | Related products: CD63 antibodies; Anti-Mouse IgG secondary antibodies; Related news: New antibodies for exosome isolation |
| Calculated MW | 26 kDa |
| PTM | Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG23346 anti-CD63 antibody [TS63] (azide free) WB image
Western blot: Human dermal fibroblasts stained with ARG23346 anti-CD63 antibody [TS63] (azide free) at dilution.
From Streck NT et al. J Virol (2020), doi: 10.1128/JVI.00609-20, Fig. 6A.
Specific References
Human Cytomegalovirus Utilizes Extracellular Vesicles to Enhance Virus Spread.
WB / Human
