ARG41312

anti-CD63 antibody [MX-49.129.5]

Datasheet

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50 μg

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Datasheet
Publication (1)

Overview

Product Description	Mouse Monoclonal antibody [MX-49.129.5] recognizes CD63
Tested Reactivity	Hu, Ms
Tested Application	FACS, ICC/IF, IHC-P, WB
Host	Mouse
Clonality	Monoclonal
Clone	MX-49.129.5
Isotype	IgG1, kappa
Target Name	CD63
Antigen Species	Human
Immunogen	Full length Human CD63.
Conjugation	Un-conjugated
Alternate Names	Tspan-30; CD63 antigen; Tetraspanin-30; CD antigen CD63; Lysosomal-associated membrane protein 3; OMA81H; Ocular melanoma-associated antigen; Granulophysin; TSPAN30; Melanoma-associated antigen ME491; MLA1; LAMP-3; ME491

Application Instructions

Application Suggestion

Tested Application	Dilution
FACS	1 - 2 µg/10^6 cells
ICC/IF	1 - 5 µg/ml
IHC-P	1 - 5 µg/ml
WB	1 - 2 µg/ml

Application Note

IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Purification with Protein G.
Buffer	PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA.
Preservative	0.05% Sodium azide
Stabilizer	0.1 mg/ml BSA
Concentration	0.2 mg/ml
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 12512 Mouse CD63 GeneID: 967 Human CD63 Swiss-port # P08962 Human CD63 antigen Swiss-port # P41731 Mouse CD63 antigen
Gene Symbol	CD63
Gene Full Name	CD63 molecule
Background	The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]
Function	Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli. [UniProt]
Cellular Localization	Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Endosome, multivesicular body. Melanosome. Secreted, exosome. Cell surface. Note=Also found in Weibel-Palade bodies of endothelial cells (PubMed:10793155). Located in platelet dense granules (PubMed:7682577). Detected in a subset of pre-melanosomes. Detected on intralumenal vesicles (ILVs) within multivesicular bodies (PubMed:21962903). [UniProt]
Highlight	Related products: CD63 antibodies; Anti-Mouse IgG secondary antibodies; Related news: New antibodies for exosome isolation
Calculated MW	26 kDa
PTM	Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro). [UniProt]

Images (7) Click the Picture to Zoom In

ARG41312 anti-CD63 antibody [MX-49.129.5] ICC/IF image

Immunofluorescence: HeLa cells stained with Alexa Fluor 488-conjugated ARG41312 anti-CD63 antibody [MX-49.129.5] (green). F-actin filaments are labeled with Dylight 554 phalloidin (red). DAPI (blue) for nuclear staining.
ARG41312 anti-CD63 antibody [MX-49.129.5] IHC-P image

Immunohistochemistry: Paraffin-embedded Human melanoma tissue stained with ARG41312 anti-CD63 antibody [MX-49.129.5].
ARG41312 anti-CD63 antibody [MX-49.129.5] WB image

Western blot: Human spleen lysate stained with ARG41312 anti-CD63 antibody [MX-49.129.5] at 2 µg/ml dilution.
ARG41312 anti-CD63 antibody [MX-49.129.5] FACS image

Flow Cytometry: Human PBMC cells stained with PE-conjugated ARG41312 anti-CD63 antibody [MX-49.129.5] (red). Cells alone (black). Isotype control (green).
ARG41312 anti-CD63 antibody [MX-49.129.5] IHC-P image

Immunohistochemistry: Paraffin-embedded Mouse spleen tissue stained with ARG41312 anti-CD63 antibody [MX-49.129.5].
ARG41312 anti-CD63 antibody [MX-49.129.5] IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded prostate carcinoma tissue stained with ARG41312 anti-CD63 antibody [MX-49.129.5].
ARG41312 anti-CD63 antibody [MX-49.129.5] FACS image

Flow Cytometry: NIH/3T3 cells stained with PE-conjugated ARG41312 anti-CD63 antibody [MX-49.129.5] (red). Cells alone (black). Isotype control (green).