ARG42260
anti-CD62L / L-Selectin antibody [DREG56] (low endotoxin)
anti-CD62L / L-Selectin antibody [DREG56] (low endotoxin) for Flow cytometry,Functional study,IHC-Frozen sections,Immunoprecipitation,Western blot and Human
Overview
| Product Description | Azide free and low endotoxin Mouse Monoclonal antibody [DREG56] recognizes CD62L / L-Selectin |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, FuncSt, IHC-Fr, IP, WB |
| Specificity | The mouse monoclonal antibody DREG56 recognizes an extracellular epitope of CD62L / L-selectin, a 65-76 kDa cell surface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, that interacts with specific carbohydrates exposed on activated endothelial cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | DREG56 |
| Isotype | IgG1 |
| Target Name | CD62L / L-Selectin |
| Antigen Species | Human |
| Immunogen | PMA-activated Human peripheral blood leukocytes. |
| Conjugation | Un-conjugated |
| Alternate Names | Leukocyte surface antigen Leu-8; Leukocyte adhesion molecule 1; CD antigen CD62L; PLNHR; LSEL; CD62L; Leukocyte-endothelial cell adhesion molecule 1; L-selectin; LAM1; LNHR; TQ1; CD62 antigen-like family member L; gp90-MEL; Lymph node homing receptor; LYAM1; LECAM1; LEU8; LAM-1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Purification Note | 0.2 µm filter sterilized. Endotoxin level is <0.01 EU/µg of the protein. |
| Buffer | PBS |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SELL |
| Gene Full Name | selectin L |
| Background | This gene encodes a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites. Single-nucleotide polymorphisms in this gene have been associated with various diseases including immunoglobulin A nephropathy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009] |
| Function | Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells (PubMed:12403782, PubMed:28489325, PubMed:28011641). Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia (PubMed:12403782, PubMed:28011641). [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 42 kDa |
Images (2) Click the Picture to Zoom In
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ARG42260 anti-CD62L / L-Selectin antibody [DREG56] (low endotoxin) FACS image
Flow Cytometry: Human peripheral blood stained with ARG42260 anti-CD62L / L-Selectin antibody [DREG56] (low endotoxin) at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG42260 anti-CD62L / L-Selectin antibody [DREG56] (low endotoxin) FACS image
Flow Cytometry: Separation of Human CD62L positive lymphocytes (red-filled) from CD62L negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG42260 anti-CD62L / L-Selectin antibody [DREG56] (low endotoxin) at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
