anti-CD5 antibody [SQab20184]

anti-CD5 antibody [SQab20184] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human



Product Description Recombinant Rabbit Monoclonal antibody [SQab20184] recognizes CD5
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SQab20184
Isotype IgG
Target Name CD5
Antigen Species Human
Immunogen Recombinant full length protein of Human CD5 (extracellular). The immunogen contains the specific extracellular domain (D32-P372).
Conjugation Un-conjugated
Alternate Names CD antigen CD5; Lymphocyte antigen T1/Leu-1; LEU1; T-cell surface glycoprotein CD5; T1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control WB: Jurkat
IHC-P: Appendix tissue
Observed Size ~ 52 kDa


Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 921 Human CD5

Swiss-port # P06127 Human T-cell surface glycoprotein CD5

Gene Symbol CD5
Gene Full Name CD5 molecule
Background This gene encodes a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2016]
Function May act as a receptor in regulating T-cell proliferation. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 55 kDa
PTM Phosphorylated on tyrosine residues by LYN; this creates binding sites for PTPN6/SHP-1. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG66751 anti-CD5 antibody [SQab20184] WB image (Customer's Feedback)

    Western blot: 10 µg of Jurkat cell lysate stained with ARG66751 anti-CD5 antibody [SQab20184] at 1:1000 dilution.

  • ARG66751 anti-CD5 antibody [SQab20184] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human appendix tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66751 anti-CD5 antibody [SQab20184] at 18°C - 25°C for 30 minutes.

Customer's Feedback

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Review for anti-CD5 antibody [SQab20184]



Sample Loading Amount:10 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC