ARG10853
anti-CD49e / Integrin alpha 5 antibody [SNAKA51]
anti-CD49e / Integrin alpha 5 antibody [SNAKA51] for Functional study,ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [SNAKA51] recognizes CD49e / Integrin alpha 5 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FuncSt, ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SNAKA51 |
| Isotype | IgG2a, kappa |
| Target Name | CD49e / Integrin alpha 5 |
| Antigen Species | Human |
| Immunogen | Whole cells corresponding to Human CD49e. |
| Conjugation | Un-conjugated |
| Alternate Names | CD49e; CD49 antigen-like family member E; Integrin alpha-F; Fibronectin receptor subunit alpha; CD antigen CD49e; VLA-5; FNRA; VLA5A; Integrin alpha-5 |
Application Instructions
| Application Suggestion |
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| Application Note | ICC/IF: A representative lot of this antibody detected Integin alpha 5 in Primary human foreskin fibroblasts (hFF). Activity Assay: A representative lot of this antibody converts alpha5 beta1 integrin into a ligand competant form and promotes fibronectin binding. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | HeLa cells. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS and 0.02% Sodium azide. |
| Preservative | 0.02% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ITGA5 |
| Gene Full Name | integrin, alpha 5 (fibronectin receptor, alpha polypeptide) |
| Background | The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. Integrin alpha 5 and integrin alpha V chains are produced by distinct genes. [provided by RefSeq, Jan 2015] |
| Function | Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. [UniProt] |
| Calculated MW | 115 kDa |
| PTM | Proteolytic cleavage by PCSK5 mediates activation of the precursor. [UniProt] |
