ARG66228

anti-CD49c / Integrin alpha 3 antibody [ASC-1]

anti-CD49c / Integrin alpha 3 antibody [ASC-1] for CyTOF®-candidate,Flow cytometry,IHC-Frozen sections,Immunoprecipitation,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [ASC-1] recognizes CD49c / Integrin alpha 3
Tested Reactivity Hu
Tested Application CyTOF®-candidate, FACS, IHC-Fr, IP, WB
Specificity This antibody recognizes CD49c (integrin alpha 3), a transmembrane glycoprotein composed of disulfide linked 125 kDa and 30 kDa chains, and expressed on adherent cell lines and to a lesser extent on T and B cells and monocytes.
Host Mouse
Clonality Monoclonal
Clone ASC-1
Isotype IgG1
Target Name CD49c / Integrin alpha 3
Antigen Species Human
Immunogen Human SSC-9 cell line (squamous cell carcinoma).
Conjugation Un-conjugated
Alternate Names FRP-2; MSK18; GAPB3; CD49 antigen-like family member C; CD49C; ILNEB; Integrin alpha-3; VL3A; Galactoprotein B3; VLA3a; CD antigen CD49c; VCA-2; VLA-3 subunit alpha; GAP-B3

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS1 - 4 µg/ml
IHC-FrAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide.
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3675 Human ITGA3

Swiss-port # P26006 Human Integrin alpha-3

Gene Symbol ITGA3
Gene Full Name integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)
Background The protein encoded by this gene belongs to the family of integrins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain, and function as cell surface adhesion molecules. This gene encodes alpha 3 subunit, which undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 subunit to form an integrin that interacts with many extracellular-matrix proteins. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008]
Function Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. [UniProt]
Highlight Related products:
CD49c antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
CyTOF-candidate Antibodies
Calculated MW A transmembrane glycoprotein composed of disulfide linked 125 kDa and 30 kDa chains.
PTM Isoform 1, but not isoform 2, is phosphorylated on serine residues. Phosphorylation increases after phorbol 12-myristate 13-acetate stimulation. [UniProt]

Images (3) Click the Picture to Zoom In

  • ARG66228 anti-CD49c / Integrin alpha 3 antibody [ASC-1] FACS image

    Flow Cytometry: HeLa cells stained with ARG66228 anti-CD49c / Integrin alpha 3 antibody [ASC-1].

  • ARG66228 anti-CD49c / Integrin alpha 3 antibody [ASC-1] WB image

    Western blot: Caco2 cells covered with matrigel for 0 or 7 days (Lane 1 & Lane 2) and Integrin alpha 3 overexpressing Caco2 cells. The blots were stained with ARG66228 anti-CD49c / Integrin alpha 3 antibody [ASC-1].

  • ARG66228 anti-CD49c / Integrin alpha 3 antibody [ASC-1] IP image

    Immunoprecipitation: Biotinylated SCC9 cell extract were immunoprecipitated by control mouse IgG (Lane 1) or ARG66228 anti-CD49c / Integrin alpha 3 antibody [ASC-1] (Lane 2 & Lane 3). Then run SDS-PAGE at non-reducing condition (Lane 2) or reducing condition (Lane 3).