ARG57601
anti-CD49b / Integrin alpha 2 antibody
anti-CD49b / Integrin alpha 2 antibody for Flow cytometry,ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CD49b / Integrin alpha 2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CD49b / Integrin alpha 2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide derived from Human Integrin alpha 2. |
| Conjugation | Un-conjugated |
| Alternate Names | Collagen receptor; VLA-2 subunit alpha; HPA-5; CD49B; CD49 antigen-like family member B; GPIa; VLA-2; CD antigen CD49b; BR; VLAA2; Platelet membrane glycoprotein Ia; Integrin alpha-2 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Microwave (8 min, twice) tissue section in Sodium citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ITGA2 |
| Gene Full Name | integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor) |
| Background | This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
| Function | Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. [UniProt] |
| Cellular Localization | Cytoplasm |
| Calculated MW | 129 kDa |
Images (2) Click the Picture to Zoom In
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ARG57601 anti-CD49b / Integrin alpha 2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse kidney tissue. Antigen Retrieval: Tissue section in Sodium citrate buffer (pH 6.0) heated in 900W for 8 min and heated again for 8 min at 750W. The tissue section was stained with ARG57601 anti-CD49b / Integrin alpha 2 antibody at 1:50 dilution, overnight at 4°C.
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ARG57601 anti-CD49b / Integrin alpha 2 antibody WB image
Western blot: A431 cell lysate stained with ARG57601 anti-CD49b / Integrin alpha 2 antibody.
