ARG62856

Name: anti-CD45 antibody [MEM-28]
Brand: arigo Biolaboratories
SKU: ARG62856
Price: 389 USD
Availability: InStock
Rating: 3.0 (1 reviews)

anti-CD45 antibody [MEM-28]

Datasheet

Ordering Information

Package

Price (USD)

100 μg

389.00

50 μg

272.00

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Overview

Product Description	Mouse Monoclonal antibody [MEM-28] recognizes CD45
Tested Reactivity	Hu
Species Does Not React With	Hrs
Tested Application	FACS, ICC/IF, IHC-P, IP, WB
Specificity	The clone MEM-28 reacts with all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets. HLDA III; WS Code NL 833a
Host	Mouse
Clonality	Monoclonal
Clone	MEM-28
Isotype	IgG1
Target Name	CD45
Antigen Species	Human
Immunogen	Human thymocytes and T lymphocytes.
Conjugation	Un-conjugated
Alternate Names	LY5; GP180; Receptor-type tyrosine-protein phosphatase C; CD45; L-CA; CD antigen CD45; Leukocyte common antigen; CD45R; LCA; T200; EC 3.1.3.48; B220

Application Instructions

Application Suggestion

Tested Application	Dilution
FACS	1 µg/ml
ICC/IF	10 µg/ml
IHC-P	10 µg/ml
IP	Assay-dependent
WB	1 µg/ml

Application Note

WB: Positive control: JURKAT human leukemia T-cell lysateKg-1a human leukemia cell lysate Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS Application note: Non-reducing conditions. SDS-PAGE (6% separating gel).
Immunohistochemistry (paraffin sections): No pre-treatment of tissue sections is essential.
Immunocytochemistry: Paraformaldehyde fixation can be used.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Purified by protein A
Purity	> 95% (by SDS-PAGE)
Buffer	PBS (pH 7.4) and 15 mM Sodium azide
Preservative	15 mM Sodium azide
Concentration	1 mg/ml
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 5788 Human PTPRC Swiss-port # P08575 Human Receptor-type tyrosine-protein phosphatase C
Gene Symbol	PTPRC
Gene Full Name	protein tyrosine phosphatase, receptor type, C
Background	CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
Function	CD45: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation. [UniProt]
Highlight	Related Antibody Duos and Panels: ARG30306 Pro-B Cell Marker Antibody panel (CD19, CD34, CD38, CD40, CD45) (FACS) ARG30307 Pre-B Cell Marker Antibody Panel (CD19, CD25, CD38, CD40, CD45) (FACS) Related products: CD45 antibodies; CD45 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Lymphoma
Research Area	Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody; Mouse Inflammatory Cell Marker antibody; B Cell Marker antibody
Calculated MW	147 kDa
PTM	Heavily N- and O-glycosylated.

Images (3) Click the Picture to Zoom In

ARG62856 anti-CD45 antibody [MEM-28] IHC-P image

Immunohistochemistry: Paraffin-embedded Human small intestine tissue stained with ARG62856 anti-CD45 antibody [MEM-28] at 10 µg/ml dilution.
ARG62856 anti-CD45 antibody [MEM-28] ICC/IF image

Immunofluorescence: Human peripheral blood mononuclear cell stained with ARG62856 anti-CD45 antibody [MEM-28] (green).
Cell nuclei was stained with DAPI (blue).
ARG62856 anti-CD45 antibody [MEM-28] FACS image

Flow Cytometry: Human peripheral blood cells stained with ARG62856 anti-CD45 antibody [MEM-28], followed by incubation with APC labelled Goat anti-Mouse secondary antibody.