ARG53851

anti-CD44 antibody [MEM-263] (APC)

Datasheet

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100 tests

389.00

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Overview

Product Description	APC-conjugated Mouse Monoclonal antibody [MEM-263] recognizes CD44
Tested Reactivity	Hu, Dog, Pig
Tested Application	FACS
Specificity	The clone MEM-263 reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. HLDA III; WS Code T 155
Host	Mouse
Clonality	Monoclonal
Clone	MEM-263
Isotype	IgG1
Target Name	CD44
Immunogen	COS-7 cells (African Green Monkey).
Conjugation	APC
Alternate Names	MDU2; MDU3; GP90 lymphocyte homing/adhesion receptor; Hermes antigen; Extracellular matrix receptor III; PGP-I; Epican; CDW44; Phagocytic glycoprotein 1; Pgp1; HUTCH-I; MC56; Hyaluronate receptor; CD antigen CD44; Heparan sulfate proteoglycan; CD44 antigen; LHR; IN; HCELL; Phagocytic glycoprotein I; PGP-1; CSPG8; MIC4; ECMR-III; CDw44

Application Instructions

Application Suggestion

Tested Application	Dilution
FACS	10 µl / 10^6 cells

Application Note

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification Note	The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer	PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative	15 mM Sodium azide
Stabilizer	0.2% (w/v) high-grade protease free BSA
Storage Instruction	Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 960 Human CD44 Swiss-port # P16070 Human CD44 antigen
Background	CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.
Research Area	Cancer antibody; Developmental Biology antibody; Immune System antibody; Chondrogenesis Study antibody
Calculated MW	82 kDa
PTM	Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. N- and O-glycosylated. O-glycosylation contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638. Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.

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ARG53851 anti-CD44 antibody [MEM-263] (APC) FACS image

Flow Cytometry: Human peripheral blood stained with ARG53851 anti-CD44 antibody [MEM-263] (APC).