ARG21352

anti-CD42b antibody [MM2/174]

anti-CD42b antibody [MM2/174] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [MM2/174] recognizes CD42b
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB
Specificity Human CD42b.
Host Mouse
Clonality Monoclonal
Clone MM2/174
Isotype IgG1, kappa
Target Name CD42b
Antigen Species Human
Immunogen Human plasma membrane
Conjugation Un-conjugated
Alternate Names CD antigen CD42b; Antigen CD42b-alpha; DBPLT3; VWDP; CD42B; GP-Ib alpha; Glycoprotein Ibalpha; BDPLT1; BSS; CD42b-alpha; GPIbA; GPIb-alpha; Platelet glycoprotein Ib alpha chain; BDPLT3; GP1B

Application Instructions

Application Suggestion
Tested Application Dilution
FACS< 1 µg/10^6 cells
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer BBS (pH 8.2)
Concentration 0.1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2811 Human GP1BA

Swiss-port # P07359 Human Platelet glycoprotein Ib alpha chain

Gene Symbol GP1BA
Gene Full Name glycoprotein Ib (platelet), alpha polypeptide
Background Glycoprotein Ib (GP Ib) is a platelet surface membrane glycoprotein composed of a heterodimer, an alpha chain and a beta chain, that is linked by disulfide bonds. The Gp Ib functions as a receptor for von Willebrand factor (VWF). The complete receptor complex includes noncovalent association of the alpha and beta subunits with platelet glycoprotein IX and platelet glycoprotein V. The binding of the GP Ib-IX-V complex to VWF facilitates initial platelet adhesion to vascular subendothelium after vascular injury, and also initiates signaling events within the platelet that lead to enhanced platelet activation, thrombosis, and hemostasis. This gene encodes the alpha subunit. Mutations in this gene result in Bernard-Soulier syndromes and platelet-type von Willebrand disease. The coding region of this gene is known to contain a polymophic variable number tandem repeat (VNTR) domain that is associated with susceptibility to nonarteritic anterior ischemic optic neuropathy. [provided by RefSeq, Oct 2013]
Function GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. [UniProt]
Calculated MW 72 kDa
PTM Glycocalicin, which is approximately coextensive with the extracellular part of the molecule, is cleaved off by calpain during platelet lysis.