ARG62829
anti-CD38 antibody [HIT2]
anti-CD38 antibody [HIT2] for CyTOF®-candidate,Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Developmental Biology antibody; Immune System antibody; Metabolism antibody; Pro-B Cell Marker antibody; Pre-B Cell Marker antibody
Overview
| Product Description | Mouse Monoclonal antibody [HIT2] recognizes CD38 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | CyTOF®-candidate, FACS, IHC-Fr, IHC-P, WB |
| Specificity | The clone HIT2 reacts with CD38 (T10), a 45 kDa type II transmembrane glycoprotein strongly expressed mainly on plasma cells and activated T and B lymphocytes; it is an antigenic marker of lymphoid cells. HLDA III; WS Code T 155 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | HIT2 |
| Isotype | IgG1 |
| Target Name | CD38 |
| Antigen Species | Human |
| Immunogen | Human thymocytes in foetus |
| Conjugation | Un-conjugated |
| Alternate Names | cADPr hydrolase 1; ADPRC 1; EC 3.2.2.6; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; Cyclic ADP-ribose hydrolase 1; ADPRC1; EC 2.4.99.20; ADP-ribosyl cyclase 1; 2'-phospho-cyclic-ADP-ribose transferase; CD antigen CD38; T10; 2'-phospho-ADP-ribosyl cyclase; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Boil for 5 min. Application note: Non-reducing condition. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | WB: Raji |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by protein A |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P28907 Human ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 |
|---|---|
| Gene Symbol | CD38 |
| Gene Full Name | CD38 molecule |
| Background | CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. Another role of CD38 is provided by enzymatic activity of its extracellular part. CD38 acts as NAD+ glycohydrolase converting NAD+ into ADP-ribose, as ADP-ribosyl cyclase producing cADPR and as cADPR hydrolase, thus affecting levels of calcium-mobilizing metabolites. ADPR produced by CD38 serves as an important second messenger of neutrophil and dendritic cell migration. |
| Function | Synthesizes the second messagers cyclic ADP-ribose and nicotinate-adenine dinucleotide phosphate, the former a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. [UniProt] |
| Highlight | Related products: CD38 antibodies; CD38 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Lymphoma CyTOF-candidate Antibodies |
| Research Area | Cancer antibody; Developmental Biology antibody; Immune System antibody; Metabolism antibody; Pro-B Cell Marker antibody; Pre-B Cell Marker antibody |
| Calculated MW | 34 kDa |
Clone References
The labelling index of primitive plasma cells determines the clinical behaviour of patients with myelomatosis.
