ARG66322
anti-CD34 antibody [SQab1870]
anti-CD34 antibody [SQab1870] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab1870] recognizes CD34 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab1870 |
| Isotype | IgG |
| Target Name | CD34 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the C-terminus of CD34. |
| Conjugation | Un-conjugated |
| Alternate Names | Hematopoietic progenitor cell antigen CD34; CD antigen CD34 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P28906 Human Hematopoietic progenitor cell antigen CD34 |
|---|---|
| Gene Symbol | CD34 |
| Gene Full Name | CD34 molecule |
| Background | CD34 protein may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
| Function | CD34 is a possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. [UniProt] |
| Highlight | Related products: CD34 antibodies; CD34 ELISA Kits; CD34 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones) |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody |
| Calculated MW | 41 kDa |
| PTM | Highly glycosylated. Phosphorylated on serine residues by PKC. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG66322 anti-CD34 antibody [SQab1870] FACS image
Flow Cytometry: Jurkat cells were fixed with 4% paraformaldehyde for 10 mins. The cells were stained with ARG66322 anti-CD34 antibody [SQab1870] (red) at 1:200 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
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ARG66322 anti-CD34 antibody [SQab1870] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded placenta tissue stained with ARG66322 anti-CD34 antibody [SQab1870] at 1:1,600 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
